{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T05:35:11Z","timestamp":1792128911689,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960746"],"award-info":[{"award-number":["81960746"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960795"],"award-info":[{"award-number":["81960795"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Studies suggest that mangiferin (MAF) has good therapeutic effects on chronic bronchitis and hepatitis. Also, it is one of the antiviral ingredients in\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    Bunge. However, its effect on the LPS\u2010induced inflammation and intestinal flora during sepsis remains unclear yet. In the present study, LPS\u2010stimulated inflammation RAW264.7 cells and LPS\u2010induced sepsis mice were used to evaluate the efficacy of MAF in\u00a0vitro and in\u00a0vivo. 16S rDNA sequencing was performed to analyze the characteristics of intestinal flora of the sepsis mice. It has been demonstrated that MAF (12.5 and 25\u2009\u03bcg\/mL) significantly inhibited protein expressions of TLR4, MyD88, NF\u2010\u03baB, and TNF\u2010\u03b1 in the LPS\u2010treated cells and reduced the supernatant TNF\u2010\u03b1 and IL\u20106 levels. In\u00a0vivo, MAF (20\u2009mg\/kg) markedly protected the sepsis mice and reduced the serum TNF\u2010\u03b1 and IL\u20106 levels. Also, MAF significantly downregulated the protein expressions of TLR4, NF\u2010\u03baB, and MyD88 in the livers. Importantly, MAF significantly attenuated the pathological injuries of the livers and small intestines. Further, MAF significantly increased proportion of\n                    <jats:italic>Bacteroidota<\/jats:italic>\n                    and decreased the proportions of\n                    <jats:italic>Firmicutes<\/jats:italic>\n                    ,\n                    <jats:italic>Desulfobacterota<\/jats:italic>\n                    ,\n                    <jats:italic>Actinobacteriota<\/jats:italic>\n                    , and\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    at phylum level, and it markedly reduced the proportions of\n                    <jats:italic>Escherichia\u2010Shigella<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudoalteromonas<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    at genus level. Moreover, MAF affects some metabolism\u2010related pathways such as citrate cycle (TCA cycle), lipoic acid metabolism, oxidative phosphorylation, bacterial chemotaxis, fatty acid biosynthesis, and peptidoglycan biosynthesis of the intestinal flora. Thus, it can be concluded that MAF as a treatment reduces the inflammatory responses in\u00a0vitro and in\u00a0vivo by inhibiting the TLR4\/ MyD88\/NF\u2010\u03baB pathway, and corrects intestinal flora imbalance during sepsis to some degree.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.3907","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T02:10:25Z","timestamp":1703729425000},"page":"2068-2080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Regulatory effects of mangiferin on\n                    <scp>LPS<\/scp>\n                    \u2010induced inflammatory responses and intestinal flora imbalance during sepsis"],"prefix":"10.1002","volume":"12","author":[{"given":"Bo\u2010tao","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Postgraduate Guizhou University of Traditional Chinese Medicine  Guiyang 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