{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T02:47:34Z","timestamp":1793242054388,"version":"4.5.0"},"reference-count":66,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Evechinus chloroticus<\/jats:italic> (commonly known as kina) is a sea urchin species endemic to New Zealand. Its roe is a culinary delicacy to the indigenous M\u0101ori and a globally exported food product. Echinochrome A (Ech A) is a bioactive compound isolated from the waste product of kina shells and spines; however, the molecular mechanisms of Ech A bioactivity are not well understood, partly due to Ech A never being studied using unbiased genome\u2010wide analysis. To explore the high\u2010value pharmaceutical potential of kina food waste, we obtained unbiased functional genomic and proteomic profiles of yeast cells treated with Echinochrome A. Abundance was measured for 4100 proteins every 30\u2009min for four hours using fluorescent microscopy, resulting in the identification of 92 proteins with significant alterations in protein abundance caused by Ech A treatment that were over\u2010represented with specific changes in DNA replication, repair and RNA binding after 30\u2009min, followed by specific changes in the metabolism of metal ions (specifically iron and copper) from 60\u2013240\u2009min. Further analysis indicated that Ech A chelated iron, and that iron supplementation negated the growth inhibition caused by Ech A. Via a growth\u2010based genome\u2010wide analysis of 4800 gene deletion strains, 20 gene deletion strains were sensitive to Ech A in an iron\u2010dependent manner. These genes were over\u2010represented in the cellular response to oxidative stress, suggesting that Ech A suppressed growth inhibition caused by oxidative stress. Unexpectedly, genes integral to cardiolipin and inositol phosphate biosynthesis were required for Ech A bioactivity. Overall, these results identify genes, proteins, and cellular processes mediating the bioactivity of Ech A. Moreover, we demonstrate unbiased genomic and proteomic methodology that will be useful for characterizing bioactive compounds in food and food waste.<\/jats:p>","DOI":"10.1002\/fsn3.4140","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T13:25:00Z","timestamp":1712064300000},"page":"4927-4943","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multi\u2010omic analysis reveals genes and proteins integral to bioactivity of Echinochrome A isolated from the waste stream of the sea urchin industry in Aotearoa New Zealand"],"prefix":"10.1002","volume":"12","author":[{"given":"Joseph","family":"Hammond","sequence":"first","affiliation":[{"name":"School of Biological Sciences Victoria University of Wellington  Wellington New\u00a0Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella M.","family":"Das","sequence":"additional","affiliation":[{"name":"Wellington High School  Wellington New\u00a0Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihana","family":"Paenga","sequence":"additional","affiliation":[{"name":"Hikurangi Bioactives Limited Partnership  Ruat\u014dria New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu","family":"Caddie","sequence":"additional","affiliation":[{"name":"Hikurangi Bioactives Limited Partnership  Ruat\u014dria New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian","family":"Skinner","sequence":"additional","affiliation":[{"name":"Hikurangi Bioactives Limited Partnership  Ruat\u014dria New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey P.","family":"Sheridan","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Victoria University of Wellington  Wellington New\u00a0Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"Miller","sequence":"additional","affiliation":[{"name":"Cawthron Institute  Nelson New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3033-395X","authenticated-orcid":false,"given":"Andrew B.","family":"Munkacsi","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Victoria University of Wellington  Wellington New\u00a0Zealand"},{"name":"Centre for Biodiscovery Victoria University of Wellington  Wellington New\u00a0Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf00369386"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9051494"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/360438"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1mb05175j"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2np00083k"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr1029"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20090555"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.04.051"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20110722"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2018.04.012"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/md16120509"},{"key":"e_1_2_10_13_1","unstructured":"Fisheries Infosite: Catch analysis of kina in New Zealand. (2022).Fisheries New Zealand. Retrieved May 19 2023 fromhttps:\/\/fs.fish.govt.nz\/Page.aspx?pk=7&tk=100&sc=SUR"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010021\u201000233\u20101"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/15648265100312s206"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1359\u20106446(03)02713\u20102"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1150021"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/md18070373"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.08.046"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02026"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/c5ay02278a"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/md12052922"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/md12084602"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010020\u201000324\u20108"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfm226"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19080412"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424\u2010014\u20101648\u20102"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20120729"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17090501"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/md21020078"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20177551"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw377"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.12.076"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.10.007"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10517\u2010015\u20102960\u20103"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1250217"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/md12063560"},{"key":"e_1_2_10_38_1","first-page":"171","article-title":"Amelioration of endotoxin\u2010induced uveitis treated with the sea urchin pigment echinochrome in rats","volume":"20","author":"Lennikov A.","year":"2014","journal-title":"Molecular Vision"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/foy040"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2005970"},{"issue":"5","key":"e_1_2_10_41_1","first-page":"Iv390","article-title":"Iron chelation by deferoxamine inhibits lipid peroxidation during cardiopulmonary bypass in humans","volume":"82","author":"Menasch\u00e9 P.","year":"1990","journal-title":"Circulation"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/md18110550"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2020.1737152"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.03.007"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2100880118"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b01285"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1604047113"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19050237"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.040"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/md21040222"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2019.194414"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19050267"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261381198"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13729"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelectrocard.2015.01.003"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/md13095722"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19100550"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.091710"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20120756"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b13274"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/md21010052"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2549"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt400"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19110622"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd6919"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/foaa050"}],"container-title":["Food Science &amp; Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.4140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T09:43:19Z","timestamp":1721814199000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/fsn3.4140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,2]]},"references-count":66,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/fsn3.4140"],"URL":"https:\/\/doi.org\/10.1002\/fsn3.4140","archive":["Portico"],"relation":{},"ISSN":["2048-7177","2048-7177"],"issn-type":[{"value":"2048-7177","type":"print"},{"value":"2048-7177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,2]]},"assertion":[{"value":"2023-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}