{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:33:19Z","timestamp":1787668399930,"version":"build-2736575974"},"reference-count":63,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001321","name":"National Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001321","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001325","name":"Cancer Association of South Africa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001325","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The anti\u2010cancer potential of\n                    <jats:italic>Cyclopia<\/jats:italic>\n                    species (honeybush) has been demonstrated in several models. The present study investigated the effects of aqueous and polyphenol\u2010enriched (PE) extracts of\n                    <jats:italic>C. subternata<\/jats:italic>\n                    and\n                    <jats:italic>C. genistoides<\/jats:italic>\n                    , as well as mangiferin and hesperidin, on different cell growth parameters in human liver (HepG2) and colon (HT\u201029) cancer cells. Mangiferin and hesperidin were most abundant in\n                    <jats:italic>C. genistoides<\/jats:italic>\n                    and\n                    <jats:italic>C. subternata<\/jats:italic>\n                    , respectively.\n                    <jats:italic>Cyclopia subternata<\/jats:italic>\n                    extracts had the highest ferric\u2010reducing antioxidant capacity. Following exposure of the cells to the extracts and compounds, cell viability, proliferation, and death (apoptosis and autophagy) were determined.\n                    <jats:italic>Cyclopia subternata<\/jats:italic>\n                    extracts reduced cell viability and inhibited cell proliferation the most, associated with depletion of ATP. In HepG2 cells, the PE extracts were less effective than the aqueous extracts in reducing cell viability but more effective in inhibiting cell proliferation. Despite disrupting cell growth, none of the extracts induced apoptosis. The aqueous extracts affected autophagy in both cancer cells. Disruption of mitochondrial membrane integrity by the different extracts, presumably via polyphenol\/iron interactions, is postulated to be involved; however, mangiferin and hesperidin had no effect, suggesting that other polyphenols and\/or complex interactions between compounds are likely responsible for the differential cytotoxic and\/or cytoprotective effects of the extracts.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.4214","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T22:33:35Z","timestamp":1715812415000},"page":"5647-5662","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Autophagy\u2010induced cell death by aqueous and polyphenol\u2010enriched extracts of honeybush (\n                    <i>Cyclopia<\/i>\n                    spp.) in\u00a0liver and colon cancer cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Sedicka","family":"Samodien","sequence":"first","affiliation":[{"name":"Applied Microbial and Health Biotechnology Institute Cape Peninsula University of Technology  Bellville South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryna","family":"de Kock","sequence":"additional","affiliation":[{"name":"Department of Medical Bioscience Program University of Western Cape  Bellville South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Joubert","sequence":"additional","affiliation":[{"name":"Plant Bioactives Group, Post\u2010Harvest &amp; Agro\u2010Processing Technologies Agricultural Research Council, Infruitec\u2010Nietvoorbij  Stellenbosch South Africa"},{"name":"Department of Food Science Stellenbosch University  Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3680-142X","authenticated-orcid":false,"given":"Dalene","family":"de Beer","sequence":"additional","affiliation":[{"name":"Plant Bioactives Group, Post\u2010Harvest &amp; Agro\u2010Processing Technologies Agricultural Research Council, Infruitec\u2010Nietvoorbij  Stellenbosch South Africa"},{"name":"Department of Food Science Stellenbosch University  Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurgen","family":"Kriel","sequence":"additional","affiliation":[{"name":"Central Analytical Facilities, Electron Microscopy Unit Stellenbosch University  Stellenbosch South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentzel C. 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