{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:34:15Z","timestamp":1789468455246,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:00:00Z","timestamp":1718841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSPD2024R709"],"award-info":[{"award-number":["RSPD2024R709"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The current study focuses on the effect of <jats:italic>Juniperus communis<\/jats:italic> extract on the probiotic properties of lactic acid bacteria isolated from camel milk in the region of El Oued (Algeria). Chromatographic analysis by HPLC was carried out to detect the most important compounds extracted from the plant. The total phenolic and flavonoid contents were determined using the colorimetric procedures Folin\u2013Ciocalteu and aluminum chloride. The probiotic properties were studied and evaluated in\u00a0vivo with <jats:italic>Juniperus communis<\/jats:italic> extracts after isolating strains from camel's milk and identifying them using 16S rRNA gene sequencing. Chromatographic profiles of the phenolic compounds revealed that <jats:italic>Juniperus communis<\/jats:italic> extract is rich in quercetin. After conducting chemical analyses of polyphenols and flavonoids, the results demonstrated a high content of phenolic compounds in <jats:italic>Juniperus communis<\/jats:italic> extracts (polyphenols: 103.80\u2009\u00b1\u20090.30\u2009mg GAE\/g E. flavonoids: 15.85\u2009\u00b1\u20090.80\u2009mg QE\/g E). Sequencing and phylogenetic analysis showed that the isolates belong to <jats:italic>Bacillus pumilus<\/jats:italic> and <jats:italic>Bacillus safensis<\/jats:italic> strains. The combination of <jats:italic>Juniperus communis<\/jats:italic> and <jats:italic>Bacillus safensis<\/jats:italic> restored the healthy intestine wall structure and returned the blood biochemical parameters to normal values. It was found that the mixture enhanced anti\u2010inflammatory effectiveness by reducing erythrocyte sedimentation rate and C\u2010reactive protein values. <jats:italic>Juniperus communis<\/jats:italic> has a high polyphenol and flavonoid content which can have a considerable impact on <jats:italic>Bacillus safensis<\/jats:italic> probiotic properties.<\/jats:p>","DOI":"10.1002\/fsn3.4262","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T05:40:34Z","timestamp":1718948434000},"page":"6509-6520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of <i>Juniperus communis<\/i> extract on probiotic properties of <i>Bacillus safensis<\/i> isolated from camel milk in the region of El Oued (Algeria)"],"prefix":"10.1002","volume":"12","author":[{"given":"Ikram","family":"Layadi","sequence":"first","affiliation":[{"name":"Laboratory of Biodiversity and Application of Biotechnology in the Agricultural Field, Faculty of the Sciences of Nature and Life University of El Oued  El Oued Algeria"},{"name":"Department of Biology, Faculty of Sciences of Nature and Life University of El\u2010Oued  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ammar Touhami","family":"Laiche","sequence":"additional","affiliation":[{"name":"Laboratory of Biodiversity and Application of Biotechnology in the Agricultural Field, Faculty of the Sciences of Nature and Life University of El Oued  El Oued Algeria"},{"name":"Department of Biology, Faculty of Sciences of Nature and Life University of El\u2010Oued  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Laid","family":"Tlili","sequence":"additional","affiliation":[{"name":"Laboratory of Biogeochemistry of Desert Environments Laboratory University of Ouargla  Ouargla Algeria"},{"name":"Department of Cellular and Molecular Biology, Faculty of Sciences of Nature and Life University of El\u2010Oued  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3536-6358","authenticated-orcid":false,"given":"Mohammed","family":"Messaoudi","sequence":"additional","affiliation":[{"name":"Nuclear Research Centre of Birine  Djelfa Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djilani","family":"Ghemam\u00a0Amara","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Sciences of Nature and Life University of El\u2010Oued  El Oued Algeria"},{"name":"Laboratory of Biology, Environment and Health, Department of Biology, Faculty of Life and Natural Sciences University of El Oued  El Oued Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maha","family":"Mezghani\u2010Khemakhem","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Biotechnology (LR01ES05), Department of Biology, Faculty of Sciences of Tunis University of Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chahnez","family":"Naccache","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Biotechnology (LR01ES05), Department of Biology, Faculty of Sciences of Tunis University of Tunis El Manar  Tunis Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Sawicka","sequence":"additional","affiliation":[{"name":"Department of Plant Production Technology and Commodities Science University of Life Sciences in Lublin  Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Atanassova","sequence":"additional","affiliation":[{"name":"Scientific Consulting, Chemical Engineering University of Chemical Technology and Metallurgy  Sofia Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wafa","family":"Zahnit","sequence":"additional","affiliation":[{"name":"Laboratory of 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