{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:53:44Z","timestamp":1792518824353,"version":"4.5.0"},"reference-count":77,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Juglans regia<\/jats:italic>\n                    L. is a well\u2010known therapeutic plant in Nepal, employed in traditional medicine for treating liver ailments. This study aimed to evaluate the in\u00a0vivo and in silico liver\u2010protective effects of\n                    <jats:italic>J. regia<\/jats:italic>\n                    extract using a carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )\u2010induced hepatic damage rat model. Healthy male rats were randomly divided into six groups: normal control (distilled water 10\u2009mL\/kg), toxic control (distilled water 10\u2009mL\/kg), standard test (silymarin 100\u2009mg\/kg), and three groups receiving oral\n                    <jats:italic>J. regia<\/jats:italic>\n                    extracts (125, 250, and 500\u2009mg\/kg\/day) for seven days. On the eighth day, carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    ) was administered intraperitoneally (i.p.) (1.5\u2009mL\/kg in 1:1 olive oil ratio for all groups, except the normal control). Rats were sacrificed on the ninth day, and blood was collected retro\u2010orbitally for liver blood injury tests and histopathological studies. Molecular docking was performed against cytochrome P450 2E1 (CYP450 2E1) enzyme for 16 selected phytoconstituents.\n                    <jats:italic>J. regia<\/jats:italic>\n                    , at doses of 125, 250, and 500\u2009mg\/kg, significantly reduced liver enzyme levels (alanine aminotransferase, alkaline phosphatase, direct bilirubin, and total bilirubin), while increasing serum albumin. Histological analysis revealed mitigation of carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )\u2010induced liver injury, reducing fatty degeneration and necrosis. Molecular docking supported the findings, with Beta\u2010sitosterol and Betulinic acid exhibiting the best binding affinity of \u22129.2 and\u2009\u22129.1 kcal\/mol, respectively. In conclusion, result suggests that\n                    <jats:italic>J. regia<\/jats:italic>\n                    showed dose\u2010dependent hepatoprotective activity in CCl\n                    <jats:sub>4<\/jats:sub>\n                    \u2010induced hepatotoxicity and it could be utilized as a promising hepatoprotective agent. This study suggests the hepatoprotective potential of\n                    <jats:italic>J. regia<\/jats:italic>\n                    bark extracts, emphasizing the need for further clinical validation.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.4288","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T05:35:56Z","timestamp":1718688956000},"page":"6482-6497","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Hepatoprotective effects of\n                    <i>Juglans regia<\/i>\n                    on carbon tetrachloride\u2010induced hepatotoxicity: In silico\/in vivo approach"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1123-2806","authenticated-orcid":false,"given":"Bipindra","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Pharmacy Madan Bhandari Academy of Health Sciences  Hetauda Nepal"},{"name":"School of Health and Allied Sciences Pokhara University  Pokhara Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1801-5668","authenticated-orcid":false,"given":"Shankar","family":"Thapa","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Madan Bhandari Academy of Health Sciences  Hetauda Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2162-2752","authenticated-orcid":false,"given":"Atisammodavardhana","family":"Kaundinnyayana","sequence":"additional","affiliation":[{"name":"School of Health and Allied Sciences Pokhara University  Pokhara Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8401-4471","authenticated-orcid":false,"given":"Sushil","family":"Panta","sequence":"additional","affiliation":[{"name":"School of Health and Allied Sciences Pokhara University  Pokhara Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.9734\/BJI\/2018\/39528"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.56499\/jppres23.1736_12.2.193"},{"key":"e_1_2_12_4_1","volume-title":"Screening of some traditionally used plants for their hepatoprotective effect","author":"Alqasoumi S. 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