{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:33:44Z","timestamp":1768264424092,"version":"3.49.0"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,21]],"date-time":"2024-07-21T00:00:00Z","timestamp":1721520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Iron deficiency anemia is a global health concern, affecting around 2\u2009billion people. Oral iron therapy often causes severe gastro\u2010intestinal issues. <jats:italic>Eruca sativa<\/jats:italic>, member of the Brassicaceae family, is valued in traditional medicine and renowned for its rich iron and vitamin C content. This study aims to evaluate the anti\u2010anemic properties of <jats:italic>E. sativa<\/jats:italic> extract in\u00a0vivo and identify its compounds targeting anemia mechanisms using network pharmacology. Thirty\u2010two Sprague\u2013Dawley rats (200\u2009\u00b1\u2009250\u2009g) were split into two distinct groups, iron\u2010deficient and iron\u2010sufficient. Three different doses (200, 400, and 800\u2009mg\/kg) of aqueous extract of <jats:italic>E. sativa<\/jats:italic> were checked against anemia by studying hematological, oxidative stress, and histopathological parameters. GC\u2013MS analysis of <jats:italic>E. sativa<\/jats:italic> revealed its phytochemical profile, followed by ADME screening. Network pharmacology explored targets related to iron deficiency anemia, with oral bioavailability and drug likeness assessment for compounds. The administration of extracts significantly improved various blood parameters, including osmotic fragility, Hb, RBCs, MCV, PCV, and alkaline phosphatase; catalase activity; and histopathological parameters such as liver in both iron\u2010deficient and iron\u2010sufficient rats (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001). Seventy\u2010nine compounds were identified in <jats:italic>E. sativa<\/jats:italic> aqueous extract, with only six of them found to be bioavailable and drug\u2010like against multiple targets. Gene ontology and pathway analysis revealed their diverse molecular, biological, and cellular functions. One gene EGFR was found to have functional association with ID anemia, suggesting potential for using <jats:italic>E. sativa<\/jats:italic> extracts. The study concludes that <jats:italic>E. sativa<\/jats:italic> extract has potential for iron deficiency anemia treatment, offering hope for future pharmaceutical interventions.<\/jats:p>","DOI":"10.1002\/fsn3.4314","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T03:29:45Z","timestamp":1721618985000},"page":"7331-7346","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010anemic potential of <i>Eruca sativa<\/i> L. in iron\u2010deficient rat model; network pharmacology profiling"],"prefix":"10.1002","volume":"12","author":[{"given":"Sana","family":"Javed","sequence":"first","affiliation":[{"name":"Department of Botany Lahore College for Women University  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zainab","family":"Shahzadi","sequence":"additional","affiliation":[{"name":"Department of Botany Lahore College for Women University  Lahore 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