{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:52:55Z","timestamp":1753869175154,"version":"3.41.2"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T00:00:00Z","timestamp":1735603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Chronic inflammation and heme\u2010iron overload can result from bacterial hemolysis. Along with the synthetic drugs, numerous traditional and functional food approaches are equally trialed to eradicate the problem. As a prospective new source of dietary protein hydrolysates, freshwater mollusks (<jats:italic>Paludomas conica<\/jats:italic>) have recently drawn huge interest from researchers. In this research, protein hydrolysate (PhPC) of <jats:italic>Paludomas conica<\/jats:italic>, prepared by the enzyme digestion method, was analyzed for proximate nutritional and minerals contents and deciphered its suppressive effects on inflammatory gene expression in LPS\u2010stimulated RAW264.7 macrophage cells. The inhibitory action of protein denaturation is also unfolded with established in\u00a0vitro and in\u00a0vivo models. Anti\u2010hemolytic, antibacterial, and thrombolytic effects of PhPC were respectively assessed by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced hemolysis of RBCs, the disc diffusion method, and the clot lysis method. The proximate nutritional and mineral contents of PhPC revealed it to be an enriched source of nutrients, crude protein, carbohydrates, Calcium, and Magnesium. Heavy metals were found to be within the prescribed limit. The PhPC suppressed the expression of inflammatory genes, including <jats:italic>COX\u20102<\/jats:italic>, <jats:italic>iNOS<\/jats:italic>, <jats:italic>IL\u20106<\/jats:italic>, <jats:italic>TNF<\/jats:italic>\u2010\u03b1, and IL\u20101, multifold in LPS\u2010stimulated RAW264.7 macrophages. The inhibition concentrations (IC<jats:sub>50<\/jats:sub>) of PhPC in the bovine serum albumin denaturation inhibition test and membrane stabilization tests were 431.39 and 285.25\u2009\u03bcg\/mL, respectively. The PhPC was discerned to be active against <jats:italic><jats:styled-content style=\"fixed-case\">Shigella flexneri<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">Pseudomonas aeruginosa<\/jats:styled-content>,<\/jats:italic> and <jats:styled-content style=\"fixed-case\"><jats:italic>Shigella dysenteriae<\/jats:italic><\/jats:styled-content>; its maximum thrombolytic effect was displayed to be 23.72%\u2009\u00b1\u20092.71%. The findings demonstrate that the nutritionally enriched PhPC could be affirmed as an exciting invertebrate anti\u2010inflammatory agent Extending other biological functions needs to be further characterized with its pure protein or protein products.<\/jats:p>","DOI":"10.1002\/fsn3.4711","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T13:15:37Z","timestamp":1735650937000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nutritional, Antibacterial and Thrombolytic Prospects of Freshwater <i>Paludomas conica<\/i> Protein Hydrolysate and Its Anti\u2010Inflammatory Potential in LPS\u2010Induced RAW 264.7 Macrophage Cells"],"prefix":"10.1002","volume":"13","author":[{"given":"Tanvir Ahmed","family":"Siddique","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5117-6829","authenticated-orcid":false,"given":"Khalid Juhani","family":"Rafi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumaiya","family":"Akter","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhana Yesmin","family":"Bithy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasheda","family":"Aktar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6059-2488","authenticated-orcid":false,"given":"Asif Nadim","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mumtahina","family":"Majid","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farjana","family":"Sultana","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srabonti","family":"Saha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Chittagong  Chittagong Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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