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Despite the potentially fatal consequences for some individuals, no antiviral drugs have been approved for the treatment of this disease. Taiwan's National Health Insurance Database (NHID) holds extensive medical records for almost every resident, encompassing conventional and traditional Chinese medicine (TCM) treatments. This study aims to uncover potential TCM formulas with antiviral properties against dengue fever by analyzing NHID data. Initially, the NHID was screened to identify candidate TCM formulas. Subsequently, immunofluorescence assays were conducted to evaluate the anti\u2010dengue virus (DENV) effects of these TCM compounds. Selected TCM formulas underwent testing for cytotoxicity and anti\u2010DENV activity. The study identified 62 promising TCM formulas, focusing on those most frequently prescribed and their potential efficacy. Among these, 23 TCM formulas showed promise in preventing severe illness, with 20 subjected to further analysis for cytotoxicity and anti\u2010DENV effects. Notably, Gastrodiae Rhizoma and Pinellia Rhizoma exhibited significant anti\u2010DENV activity at different multiplicities of infection (MOIs). This research introduces a novel methodology for identifying potential antiviral compounds against dengue fever, leveraging NHID data alongside immunofluorescence assays.<\/jats:p>","DOI":"10.1002\/fsn3.70009","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T05:24:54Z","timestamp":1741757094000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessing Traditional Chinese Medicines for Anti\u2010Dengue Using a National Health Insurance Research Database and Bioassays"],"prefix":"10.1002","volume":"13","author":[{"given":"Wan\u2010Yu","family":"Su","sequence":"first","affiliation":[{"name":"School of Pharmacy National Defense Medical Center  Taipei Taiwan, ROC"},{"name":"Department of Pharmacy Tri\u2010Service General Hospital  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiang\u2010Hui","family":"Lo","sequence":"additional","affiliation":[{"name":"School of Pharmacy National Defense Medical Center  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Liang","family":"Wen","sequence":"additional","affiliation":[{"name":"School of Pharmacy National Defense Medical Center  Taipei Taiwan, ROC"},{"name":"Graduate Institute of Life Sciences National Defense Medical Center  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeng\u2010Wei","family":"Lu","sequence":"additional","affiliation":[{"name":"Biotech Research and Innovation Centre University of Copenhagen  Copenhagen Denmark"},{"name":"The Finsen Laboratory Rigshospitalet\/National University Hospital  Copenhagen Denmark"},{"name":"Department of Bioscience and Biotechnology National Taiwan Ocean University  Keelung Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u2010Chuan","family":"Yen","sequence":"additional","affiliation":[{"name":"School of Pharmacy National Defense Medical Center  Taipei Taiwan, ROC"},{"name":"Department of Pharmacy Tri\u2010Service General Hospital  Taipei Taiwan, ROC"},{"name":"Graduate Institute of Life Sciences National Defense Medical Center  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Jung","family":"Ho","sequence":"additional","affiliation":[{"name":"School of Pharmacy National Defense Medical Center  Taipei Taiwan, ROC"},{"name":"Graduate Institute of Life Sciences National Defense Medical Center  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0692-7408","authenticated-orcid":false,"given":"Li\u2010Ting","family":"Kao","sequence":"additional","affiliation":[{"name":"School of Pharmacy National Defense Medical Center  Taipei Taiwan, ROC"},{"name":"Department of Pharmacy Tri\u2010Service General Hospital  Taipei Taiwan, ROC"},{"name":"Graduate Institute of Life Sciences National Defense Medical Center  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/313842"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010020\u201000410\u20100"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14040338"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1142\/s0192415x20500160"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2012.10.013"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3277268"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.22112"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13101967"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010023\u201000797\u20107"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2007.06.021"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2010.29"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2002.1704"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/507635"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1892"},{"key":"e_1_2_10_16_1","unstructured":"WHO.2023.\u201cDengue and Severe Dengue.\u201dhttps:\/\/www.who.int\/news\u2010room\/fact\u2010sheets\/detail\/dengue\u2010and\u2010severe\u2010dengue."},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24705"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2017.12.029"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep45171"}],"container-title":["Food Science &amp; 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