{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:44:53Z","timestamp":1786977893437,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This Research Aimed to Discuss the Protective Mechanism of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lycium barbarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    Polysaccharides (\n                    <jats:styled-content style=\"fixed-case\">LBPs<\/jats:styled-content>\n                    ) Against Ethanol (\n                    <jats:styled-content style=\"fixed-case\">EtOH<\/jats:styled-content>\n                    )\u2010caused Hepatocellular Damage. Normal human hepatocytes (L\u201002 cells) were processed with 100\u2009\u03bcg\/mL EtOH to simulate liver injury, followed by treatment with LBPs at different concentrations (12, 24, 48\u2009\u03bcg\/mL) to determine the optimal dose. Cells were divided into the control, EtOH, EtOH+LBP\u2010treated, and EtOH+LBP\u2010treated with siRNA against PPAR\u2010\u03b1 groups. To evaluate treatment effects, the MTT assay was utilized for measuring cell viability, succeeded by the assessment of liver injury markers (ALT, AST, TG) and inflammatory cytokines (IL\u20101\u03b2, TNF\u2010\u03b1, and IL\u20106). Besides, the GSDMD, NLRP\u20103, caspase\u20101, and PPAR\u2010\u03b1 protein levels were analyzed via western blotting. Relative to the Control group, EtOH exposure remarkably decreased cell viability, increased TG, AST, and ALT levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), and induced cell damage and lipid accumulation. It also elevated inflammatory cytokine levels and triggered pyroptosis (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). However, LBP treatment alleviated EtOH\u2010induced damage, reduced lipid accumulation, inhibited pyroptosis\u2010related protein expression, suppressed inflammatory responses, and upregulated PPAR\u2010\u03b1 protein expression (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). LBPs can alleviate EtOH\u2010induced L\u201002 cell injury, lipid accumulation, inflammatory response, and pyroptosis. The mechanism is possibly associated with inhibiting NLRP\u20103\/caspase\u20101\u2010mediated cell pyroptosis by activating PPAR\u2010\u03b1 expression, thus protecting hepatocytes from injury.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70172","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T06:40:41Z","timestamp":1744958441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Lycium barbarum<\/i>\n                    Polysaccharides Alleviate Ethanol\u2010Induced Liver Injury by Activating\n                    <scp>PPAR<\/scp>\n                    \u2010\u03b1 and Inhibiting\n                    <scp>NLRP<\/scp>\n                    \u20103\/Caspase\u20101\u2010Mediated Pyroptosis"],"prefix":"10.1002","volume":"13","author":[{"given":"Le\u2010bin","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Infectious Disease, Guangzhou First People's Hospital, School of Medicine South China University of Technology  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, Guangzhou First People's Hospital, School of Medicine South China University of Technology  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Nursing, Guangzhou First People's Hospital, School of Medicine South China University of Technology  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6757-1121","authenticated-orcid":false,"given":"Wen\u2010zhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, Guangzhou First People's Hospital, School of Medicine South China University of Technology  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.71081\/cvj\/2024.017"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2022.11.002"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2018.07.029"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/13510002.2023.2187564"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021316"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5823823"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115717"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aohep.2023.101082"},{"key":"e_1_2_13_10_1","unstructured":"Khan A. 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