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Urea is an arginine metabolite produced by <jats:styled-content style=\"fixed-case\"><jats:italic>Saccharomyces cerevisiae<\/jats:italic><\/jats:styled-content>. Previous studies have shown that <jats:italic>BTN2<\/jats:italic> influences arginine metabolism. In this study, we compared the effects of <jats:italic>BTN2\u2010<\/jats:italic>modified strains on key metabolites, enzymes, and transcriptional gene expressions in the arginine metabolic pathway, and assessed cell growth and oxidative damage under different ethanol stresses. It revealed that the knockout of <jats:italic>BTN2<\/jats:italic> inhibited arginine intake and promoted urea reduction. RT\u2010qPCR results demonstrated that <jats:italic>BTN2<\/jats:italic> regulate arginine transportation, catabolism, and urea degradation by modulating the expression of <jats:italic>GAP1<\/jats:italic>, <jats:italic>CAN1<\/jats:italic>, <jats:italic>CAR1<\/jats:italic>, and <jats:italic>DUR1,2<\/jats:italic>. Moreover, the results showed that <jats:italic>BTN2<\/jats:italic> enhanced ethanol tolerance and alleviated cellular damage. These findings provide a promising method for reducing arginine uptake by <jats:styled-content style=\"fixed-case\"><jats:italic>Saccharomyces cerevisiae<\/jats:italic><\/jats:styled-content> and consequently urea accumulation in wine.<\/jats:p>","DOI":"10.1002\/fsn3.70244","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T12:24:48Z","timestamp":1746188688000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Regulation of Arginine Metabolism and Ethanol Tolerance in <scp><i>Saccharomyces cerevisiae<\/i><\/scp> by <scp><i>BTN2<\/i><\/scp>"],"prefix":"10.1002","volume":"13","author":[{"given":"Ting","family":"Xia","sequence":"first","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiwei","family":"Chen","sequence":"additional","affiliation":[{"name":"Youxian Shop (Zhejiang) Food Co. 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Huzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tangchao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gongnian","family":"Xiao","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3434-5843","authenticated-orcid":false,"given":"Ruosi","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering Zhejiang University of Science and Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3mt00107e"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.182.22.6418\u20106423.2000"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/ec.1.4.606\u2010612.2002"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2022.101725"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31311"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567\u20101364.2008.00397.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.12665"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.04.014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.525915"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.12.072"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295\u2010016\u20101737\u20107"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0021\u20109258(17)39106\u20108"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541\u20104337.12084"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010018\u20109313\u2010x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/foz079\/5621491"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.822864"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.137729"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00082"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002940050506"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12203816"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.2005.03501.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.132"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010021\u201011348\u20101"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25163580"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2014.04.007"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010020\u201010549\u20104"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.13375\/j.cnki.wcjps.2020.06.010"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20910"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b05348"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010018\u20102430\u2010y"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2013.05.009"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126554"}],"container-title":["Food Science &amp; 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