{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:08:29Z","timestamp":1783555709883,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M761857"],"award-info":[{"award-number":["2024M761857"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to investigate the beneficial effects of \u03b1\u2010linolenic acid (ALA) on intestinal barrier function and antioxidant status in broilers, along with the associated molecular mechanisms. 320 one\u2010day\u2010old Arbor Acres broilers were randomly divided into four groups, each with eight replicates, and fed diets with 0 (control), 200, 400, and 600\u2009mg of ALA\/kg for 42\u2009days. ALA supplementation did not significantly affect the broilers' overall growth performance. Supplementing diets with 400 and 600\u2009mg\/kg of ALA significantly enhanced (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) jejunal and ileal villus height, the jejunal villus height to crypt depth ratio, and ileal mRNA expression and protein levels of Zonula occludens\u20101 (ZO\u20101) and occludin in broilers on Day 42. Broilers fed diets containing 600\u2009mg\/kg of ALA exhibited significantly increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) serum catalase (CAT) activity, total antioxidant capacity (T\u2010AOC), and jejunal and ileal activities of CAT and total superoxide dismutase (T\u2010SOD), alongside reduced malondialdehyde (MDA) concentrations in serum, jejunum, and ileum on Days 21 and 42, compared to the control group. Supplementing 600\u2009mg\/kg of ALA significantly increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) the mRNA expressions of CAT, SOD1, NRF2, and HO\u20101, along with the protein levels of cytoplasmic and nuclear NRF2 and HO\u20101 in the jejunum and ileum on Days 21 and 42. These findings demonstrate the protective effects of ALA in improving intestinal health in broilers. The underlying mechanisms may involve enhancing intestinal barrier integrity by increasing tight junction protein abundance and boosting intestinal antioxidant capacity by elevating antioxidant enzyme activity and activating the NRF2 pathway. In conclusion, our results showed that 600\u2009mg\/kg of ALA was identified as the optimal concentration for improving intestinal barrier function and antioxidant status in broilers, highlighting its potential for protecting intestinal health through ALA\u2010based interventions.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70271","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T07:44:51Z","timestamp":1748418291000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Influence of \u03b1\u2010Linolenic Acid on the Intestinal Barrier Integrity and Intestinal Antioxidant Status in Broilers"],"prefix":"10.1002","volume":"13","author":[{"given":"Ao","family":"Kang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University  Tai'an Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialei","family":"Ni","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University  Tai'an Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University  Tai'an Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyu","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University  Tai'an Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northeast Agricultural University  Harbin Heolongjiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiming","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University  Tai'an Shandong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4831-2754","authenticated-orcid":false,"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University  Tai'an Shandong People's Republic of China"},{"name":"College of Veterinary Medicine Northeast Agricultural University  Harbin Heolongjiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2018.10.002"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00201.2017"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/33.3.590"},{"key":"e_1_2_9_5_1","first-page":"239","article-title":"The Role of Intestinal Microbial\/Immune Cell Interactions in Patients With Inflammatory Bowel Disease","volume":"22","author":"Chairakaki A. 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