{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T11:10:55Z","timestamp":1778929855456,"version":"3.51.4"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T00:00:00Z","timestamp":1748131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T00:00:00Z","timestamp":1748131200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of this study is to investigate the anticancer potential of\n                    <jats:italic>Betonica macrantha<\/jats:italic>\n                    extract on MDA\u2010MB\u2010231 breast cancer cells, its regulatory effects on miRNA expression, the content analysis of its phytochemical components, and the roles of these compounds in the regulation of miRNA expression through pathways, as well as to examine its pharmacokinetic profiles. The\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. macrantha<\/jats:italic>\n                    <\/jats:styled-content>\n                    plant was extracted with methanol. The obtained extracts were analyzed for phytochemical components using gas chromatography\u2013mass spectrometry (GC\u2013MS) and high\u2010performance liquid chromatography (HPLC\u2010DAD) techniques. Biological activity was assessed using the MTT assay on the MDA\u2010MB\u2010231 breast cancer cell line, while miRNA expression was measured by RT\u2010PCR. Pharmacokinetic properties were calculated using ADMETSAR3 software, and molecular interactions were investigated through AutoDock Vina simulations. GC\u2013MS analysis of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. macrantha<\/jats:italic>\n                    <\/jats:styled-content>\n                    extract identified 42 volatile compounds, with 1S\u2010\u03b1\u2010pinene, humulene, and caryophyllene being the most abundant. HPLC\u2010DAD analysis detected 18 bioactive compounds, including catechin, oleuropein, and rutin. The extract inhibited the viability of MDA\u2010MB\u2010231 breast cancer cells in a dose\u2010dependent manner, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 0.8\u2009mg\/mL. Furthermore, upregulation of miR\u201019, miR\u201020a, miR\u2010126, and miR\u2010200c miRNAs was observed in MDA\u2010MB\u2010231 cells, while these miRNAs were downregulated in healthy cells. ADMET analysis revealed that \u03b1\u2010pinene, caryophyllene, and catechin exhibited high bioavailability, absorption, and distribution properties, while oleuropein and rutin showed limited absorption and bioavailability. Molecular docking studies demonstrated the potential binding interactions of these compounds with key target proteins involved in cancer progression. Consequently,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. macrantha<\/jats:italic>\n                    <\/jats:styled-content>\n                    presents significant potential as a valuable natural source for cancer therapy through its anticancer activity, modulation of miRNA expression, and interaction with cancer\u2010associated proteins.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70341","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T00:15:28Z","timestamp":1748218528000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Regulatory Effects of\n                    <i>Betonica macrantha<\/i>\n                    Extract, Known as Mountain Tea, on\n                    <scp>miRNA<\/scp>\n                    Expression and Its Pharmacokinetic Properties"],"prefix":"10.1002","volume":"13","author":[{"given":"Muhammed Emin","family":"Sar\u0131","sequence":"first","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Necmettin Erbakan University  Konya T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4641-0067","authenticated-orcid":false,"given":"Emine Incilay","family":"Toruno\u011flu","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Necmettin Erbakan University  Konya T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeynep Bet\u00fcl","family":"Sar\u0131","sequence":"additional","affiliation":[{"name":"Department of Basic Medical Sciences, Medical Biology, Faculty of Medicine Ankara Y\u0131ld\u0131r\u0131m Beyaz\u0131t University  Ankara T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6045-0183","authenticated-orcid":false,"given":"Erdi Can","family":"Aytar","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Faculty of Agriculture Usak University  U\u015fak T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abidin","family":"G\u00fcmr\u00fck\u00e7\u00fco\u011flu","sequence":"additional","affiliation":[{"name":"Medicinal\u2010Aromatic Plants Application and Research Center Artvin \u00c7oruh University  Artvin T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alper","family":"Durmaz","sequence":"additional","affiliation":[{"name":"Ali Nihat G\u00f6kyi\u011fit Botanical Garden Application and Research Center Artvin \u00c7oruh University  Artvin T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gamze","family":"Demirel","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Ak\u015fehir Kadir Yallag\u00f6z School of Health Sel\u00e7uk University  Konya T\u00fcrkiye"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e36223"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13164114"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2020.173509"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11947\u2010024\u201003539\u20101"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1924265"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23040965"},{"key":"e_1_2_8_8_1","unstructured":"Biovia D.2021.\u201cDassault Syst\u00e8mes.\u201dDiscovery Studio Visualizer v21.1.0.20298."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m115.661504"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(91)83028\u2010J"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci300367a"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9020276"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2634"},{"key":"e_1_2_8_14_1","volume-title":"Flora of Turkey and the East Aegean Islands","author":"Davis P. 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