{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:42:49Z","timestamp":1786977769116,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study investigates the chemopreventive potential of a grape pomace extract (GPE) derived from Verdicchio grapes, aligning with circular economy principles to repurpose winery waste into a nutraceutical targeting gastric cancer prevention. Soxhlet extraction yielded a bioactive\u2010rich extract. Comprehensive chemical characterization via HPLC\/ESI\/Q\u2010TOF identified 39 metabolites spanning key chemical classes. Anthocyanins were predominant, with malvidin glucoside (12,546\u2009mg\/kg\u2009DM; 36.3%), malvidin coumaroyl glucoside (9941\u2009mg\/kg\u2009DM; 28.8%), and malvidin acetylglucoside (7189\u2009mg\/kg\u2009DM; 20.8%) as the most abundant compounds. Carboxylic acids included tartaric, malic, isocitric, aconitic, and succinic acids, while lipid molecules, such as phytosphingosine and stearic acid, and amino acids like proline, valine, leucine, and phenylalanine further enriched the extract's chemical heterogeneity.<\/jats:p>\n                  <jats:p>\n                    Biological evaluations revealed GPE's selective cytotoxicity against AGS (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 13.64\u2009\u03bcg\/mL) and KATO III (IC\n                    <jats:sub>50<\/jats:sub>\n                    : 7.11\u2009\u03bcg\/mL) gastric cancer cells, sparing GES\u20101 cells. Mechanistically, GPE\u2010induced apoptosis through caspase\u20103 activation and mitochondrial dysfunction, as evidenced by inner membrane disruption and cardiolipin peroxidation. TEM and CLSM morphological analyses revealed hallmark apoptotic features, including chromatin condensation, micronuclei formation, and apoptotic bodies. Additionally, GPE impaired autophagy, marked by Beclin\u20101 downregulation and LC3B\u2010II upregulation. The accumulation of degradative vacuoles indicated disrupted autophagosome clearance, shifting autophagy from a survival mechanism to a cell death\u2010promoting pathway. These findings highlight GPE's dual impact on apoptosis and autophagy in gastric cancer cells, underscoring its potential as a dietary intervention for gastric cancer prevention.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70350","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T11:59:43Z","timestamp":1748606383000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Food Waste to Wellness: A Grape Pomace Blend in Gastric Cancer Prevention"],"prefix":"10.1002","volume":"13","author":[{"given":"Matteo","family":"Micucci","sequence":"first","affiliation":[{"name":"Department of Biomolecular Sciences University of Urbino Carlo Bo  Urbino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4028-0652","authenticated-orcid":false,"given":"Michela","family":"Battistelli","sequence":"additional","affiliation":[{"name":"Department of 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