{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:44:31Z","timestamp":1792464271304,"version":"4.5.0"},"reference-count":78,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Osteoporosis, characterized by reduced bone mineral density (BMD) and impaired bone microarchitecture, imposes a significant global health and economic burden, particularly on postmenopausal women. This study investigated the efficacy of a novel supplement that combines pilose antler extract and hydroxytyrosol (Ruiling capsule) in improving bone health in animal and human trials. In an osteoporotic rat model, Ruiling supplementation significantly increased BMD and bone calcium content, particularly at medium doses, surpassing untreated controls and restoring values to levels comparable with non\u2010osteoporotic rats. In a parallel randomized, double\u2010blind, placebo\u2010controlled clinical trial, postmenopausal women who received Ruiling capsules for 32\u2009weeks showed marked improvements in lumbar\u2010spine and femoral\u2010neck BMD, bone turnover markers, antioxidant enzyme activities, and reduced low back pain compared to the placebo group. No adverse effects were observed, suggesting that modulation of oxidative stress may underpin the bone\u2010protective effects. These findings highlight the potential of combining pilose antler extract and hydroxytyrosol to address the multifactorial osteoporosis pathogenesis. The Ruiling capsule represents a safe, non\u2010pharmacological intervention that offers both structural and functional benefits and could contribute to the long\u2010term management of osteoporosis in aging populations.<\/jats:p>","DOI":"10.1002\/fsn3.70402","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T03:45:15Z","timestamp":1749699915000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Combination of Pilose Antler Extract and Hydroxytyrosol Enhances Bone Mineral Density in Both Animals and Postmenopausal Women"],"prefix":"10.1002","volume":"13","author":[{"given":"Xichi","family":"Ma","sequence":"first","affiliation":[{"name":"Bayi Orthopaedic Hospital  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Ma","sequence":"additional","affiliation":[{"name":"Bayi Orthopaedic Hospital  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xilin","family":"Ma","sequence":"additional","affiliation":[{"name":"Bayi Orthopaedic Hospital  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zubing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yiping Medical Science &amp; Technology Development Co. Ltd  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2972-2173","authenticated-orcid":false,"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"Hehui Pharmaceutical Technology Co., Ltd  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310824"},{"issue":"1","key":"e_1_2_12_3_1","first-page":"47","article-title":"Markets and the Use of Wild Animals for Traditional Medicine: A Case Study Among the Tsimane'amerindians of the Bolivian Rain Forest","volume":"23","author":"Apaza L.","year":"2003","journal-title":"Journal of Ethnobiology"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109384(76)90064\u20100"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci11066"},{"issue":"6","key":"e_1_2_12_6_1","first-page":"308","article-title":"Effects of Aging and Dietary Antler Supplementation on the Calcium\u2010Regulating Hormones and Bone Status in Ovariectomized SAMP8 Mice","volume":"50","author":"Chen C.","year":"2007","journal-title":"Chinese Journal of Physiology"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/819520"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092213"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02678146"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198\u2010020\u201005378\u20100"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198\u2010014\u20102794\u20102"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2009.01158.x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11657\u2010021\u201000958\u2010x"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0809493"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.6.953"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.69"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2017.4728"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167\u20104781(95)00106\u2010q"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0167\u20104781(96)00178\u20109"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/138527208784083888"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1340"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2015.09.010"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0031\u20109384(99)00060\u20108"},{"issue":"1367","key":"e_1_2_12_24_1","first-page":"80","article-title":"Health Benefits of Deer and Elk Velvet Antler Supplements: A Systematic Review of Randomised Controlled Studies","volume":"125","author":"Gilbey A.","year":"2012","journal-title":"New Zealand Medical Journal"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.04.023"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.120738"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2015\u20101726"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2004.06.001"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200900098"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1071\/AN11183"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(02)08761\u20105"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198\u2010018\u20104704\u20105"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650090802"},{"key":"e_1_2_12_34_1","first-page":"245","article-title":"Deer Antlers\u2010Traditional Use and Future Perspectives","volume":"9","author":"Kawtikwar P. S.","year":"2010","journal-title":"Indian Journal of Traditional Knowledge"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/61.6.1321S"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a114480"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2003.4.2.151"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2007.01.001"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15024"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2023.122066"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756\u20103282(96)00138\u2010X"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.07.091"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13002\u2010024\u201000669\u2010w"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.116052"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12369"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2009\u20100024"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejm199502023320506"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2013.179"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines5010013"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.3.7867582"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1056\/nejm200105103441904"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.173"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097\u20100177(199603)205:3<332::Aid\u2010aja12>3.0.Co;2\u20106"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf801794q"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20041181"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.34171\/mjiri.34.154"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875\u20105364(19)30092\u20105"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4020-8356-3_3"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2011.04.022"},{"key":"e_1_2_12_60_1","first-page":"1","article-title":"The Global Prevalence of Osteoporosis in the World: A Comprehensive Systematic Review and Meta\u2010Analysis","volume":"16","author":"Salari N.","year":"2021","journal-title":"Journal of Orthopaedic Surgery and Research"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.5551\/jat.47993"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(13)70045-7"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110813"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.07.044"},{"key":"e_1_2_12_65_1","doi-asserted-by":"crossref","unstructured":"Sunwoo H. H.1998.\u201cChemical Characterization of Growing Antlers From Wapiti (Cervus elaphus).\u201d","DOI":"10.1016\/S0305-0491(98)10127-X"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.4141\/a96\u2010121"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0305\u20100491(98)10127\u2010x"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719\u2010019\u201009859\u20101"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(71)90050-3"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.35.654"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044552"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774\u2010023\u201001487\u2010y"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0010(20000515)80:7<1013::AID-JSFA571>3.0.CO;2-C"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14194183"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgab180"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2019.8286"},{"key":"e_1_2_12_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2019.12.005"},{"key":"e_1_2_12_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.09.027"},{"key":"e_1_2_12_79_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242115772"}],"container-title":["Food Science &amp; Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.70402","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/fsn3.70402","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.70402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T10:43:25Z","timestamp":1778928205000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/fsn3.70402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":78,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/fsn3.70402"],"URL":"https:\/\/doi.org\/10.1002\/fsn3.70402","archive":["Portico"],"relation":{},"ISSN":["2048-7177","2048-7177"],"issn-type":[{"value":"2048-7177","type":"print"},{"value":"2048-7177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"2024-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70402"}}