{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:57:36Z","timestamp":1791388656322,"version":"4.3.3"},"reference-count":84,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100016209","name":"Bilimsel Ara\u015ft\u0131rma Projeleri, Erciyes \u00dcniversitesi","doi-asserted-by":"publisher","award":["TYL\u20102023\u201012905"],"award-info":[{"award-number":["TYL\u20102023\u201012905"]}],"id":[{"id":"10.13039\/501100016209","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>Centaurea<\/jats:italic>\n                    species (Asteraceae) is widely used in folk medicine. However, its apoptotic effects have not been adequately determined.\n                    <jats:italic>Centaurea lycaonica<\/jats:italic>\n                    is an endemic species belonging to the\n                    <jats:italic>Centaurea<\/jats:italic>\n                    L. genus. In this study, methanol (CHM) and dichloromethane (CHD) extracts obtained from the aerial part of\n                    <jats:italic>C. lycaonica<\/jats:italic>\n                    were treated to human cervical carcinoma (HeLa) cells to investigate their cytotoxic effects using Sulforhodamine B (SRB) assay and xCELLigence Real\u2010Time Cell analyzing system, in addition to their apoptotic effects by measuring caspases. As a result, the IC\n                    <jats:sub>50<\/jats:sub>\n                    value of CHD and CHM on HeLa cells was calculated respectively as 39.27\u2009\u03bcg\/mL and 516.50\u2009\u03bcg\/mL. Increased activity of caspase\u20103 and \u20109, along with a higher Bax\/Bcl\u20102 ratio, was observed in the HeLa cells at 30 and 100\u2009\u03bcg\/mL concentrations for CHD and 240\u2009\u03bcg\/mL for CHM. Significant cytotoxic effects through the mitochondrial apoptotic pathway were shown for the HeLa cells. LC\u2010HRMS determined the phytochemical content of extracts. The major compounds, diosmetin, and apigenin, were detected in the CHD extract, and chlorogenic and quinic acid were detected in the CHM extract. Apoptosis is induced primarily by CHD, which contains a high amount of diosmetin and is a promising candidate for anticancer research.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70528","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T09:51:27Z","timestamp":1752573087000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Centaurea lycaonica<\/i>\n                    Extracts Induce Apoptosis in\n                    <scp>HeLa<\/scp>\n                    Human Cervical Cancer Cells via Bax\/Bcl\u20102 Modulation and Caspase Activation: An\n                    <scp>LC<\/scp>\n                    \u2010\n                    <scp>HRMS<\/scp>\n                    \u2010Based Study"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4689-0657","authenticated-orcid":false,"given":"Ay\u015fe K\u00fcbra","family":"Karabo\u011fa Arslan","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8592-2359","authenticated-orcid":false,"given":"Safa","family":"Emino\u011flu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3860-7222","authenticated-orcid":false,"given":"Leyla","family":"Pa\u015fayeva","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2965-6719","authenticated-orcid":false,"given":"Nuh Mehmet","family":"Bozkurt","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy Erciyes University  Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3980-7648","authenticated-orcid":false,"given":"Osman","family":"Tugay","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy Sel\u00e7uk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/274207"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(95)90036-5"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.4274\/tjps.galenos.2018.27146"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472\u20106882\u20109\u201036"},{"issue":"4","key":"e_1_2_12_6_1","first-page":"149","article-title":"The Biological Activity of Centaurea L. Species","volume":"17","author":"Arif R.","year":"2004","journal-title":"Gazi University Journal of Science"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10520295.2020.1751288"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/tjb\u20102016\u20100128"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.21010\/ajtcam.v13i6.19"},{"issue":"3","key":"e_1_2_12_10_1","first-page":"103","article-title":"Chemical Composition and Cytotoxic Activity of Centaurea Scoparea Sieb Against Four Human Cell Lines","volume":"7","author":"Beltagy A. M.","year":"2015","journal-title":"Journal of Pharmaceutical Sciences and Research"},{"key":"e_1_2_12_11_1","unstructured":"Bozkurt E.1997.\u201cPharmacognostical Studies onCentaurae virgateLam.\u201dMaster Thesis Hacettepe University Institute of Health Sciences Ankara T\u00fcrkiye."},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2019.1644507"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants11233267"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616\u2010018\u20100290\u20109"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.26444\/aaem\/108563"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14652"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113568"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109091"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.3187"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2755"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/125286"},{"key":"e_1_2_12_22_1","volume-title":"Red Book of Turkish Plants","author":"Ekim T.","year":"2000"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202402768"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2010.551538"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/1386207319666151203002117"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.5530\/ax.2011.2.7"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2018.05.046"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c01819"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0378\u20108741(99)00112\u20109"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.17343\/sdutfd.619417"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2021.1955882"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871523019666200609140532"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20108741(95)01241\u20105"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010022\u201001735\u2010y"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1080\/10520295.2017.1401662"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11071053"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0277\u20105379(91)90142\u2010z"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/bios5020199"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.17691\/stm2020.12.4.13"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.08.017"},{"issue":"1","key":"e_1_2_12_41_1","first-page":"101","article-title":"Cytotoxic and Antibacterial Activities of Centaurea Cadmea Boiss","volume":"11","author":"K\u00f6ksal C. 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