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Adjusting the pH of the remaining process water after protein recovery at their isoelectric point to 8 enabled precipitation and recovery of SPs with the aid of ethanol at both alkaline and acid process versions and named SP\u201011.5 and SP\u20102.5, respectively. The mass yield of SPs recovered using the alkaline process version was 3.25%, nearly double that of SP (1.75%) from the acid version. SP\u201011.5 contained higher levels of carbohydrates (61.22%), proteins (13.29%), and sulfates (12.13%) compared to SP\u20102.5. FTIR, DSC, and XRD analyses showed no significant differences in the structural properties of the recovered SPs as a function of the pH\u2010shift process version. However, SP\u201011.5 exhibited better antioxidant activity in DPPH, ABTS, and metal chelating tests and superior antibacterial properties against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    than SP\u20102.5. This study suggests that the pH\u2010shift process can be effectively extended for sequential extraction of both protein isolates and SPs from fish by\u2010products for a multiple product biorefinery where the alkaline version outperformed.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70673","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T00:33:53Z","timestamp":1753922033000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Expanding the pH\u2010Shift Technique for Sequential Extraction of Intact Proteins and Sulfated Polysaccharide From Fish Heads: A Novel Biorefinery Approach"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8488-8742","authenticated-orcid":false,"given":"Shahab","family":"Naghdi","sequence":"first","affiliation":[{"name":"Seafood Processing Department, Marine Sciences Faculty Tarbiat Modares University  Noor 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