{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T13:37:49Z","timestamp":1764769069998,"version":"3.46.0"},"reference-count":87,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["51975458","51605370"],"award-info":[{"award-number":["51975458","51605370"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Effective hemostatic and anti\u2010inflammatory measures are essential for wound healing. In this study, Ultra\u2010performance liquid chromatography (UPLC) combined with network pharmacology was used to analyze the composition of walnut shell ethanol extract (WSEE), and in\u00a0vivo and in\u00a0vitro experiments elucidated its potential mechanisms of hemostatic and anti\u2010inflammatory effects. The results showed that 45 components were identified from WSEE, and the NF\u2010\u03baB signaling pathway, IL\u201017 signaling pathway, etc., could play significant roles in this context. In\u00a0vitro, WSEE exhibited procoagulant activity including shortened serum APTT (activated partial thromboplastin time, 40.4\u2009\u00b1\u20093.05\u2009s), TT (thrombin time, 26.13\u2009\u00b1\u20091.6\u2009s), and PT (prothrombin time, 14.6\u2009\u00b1\u20092.1\u2009s) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), and increasing FIB levels (Fibrinogen, 3.7\u2009\u00b1\u20091.01\u2009s). In\u00a0vivo, WSEE significantly shortened the clotting time in the WSEE group (05:26\u2009s) compared with the control group (13:25\u2009s). Comparing to the lipopolysaccharide alone treatment group, the expression of pro\u2010inflammatory factors such as TNF\u2010\u03b1 (61.63%, 73.06%, and 66.03%), IL\u20101\u03b2 (94.78%, 95.40%, and 88.99%), and IL\u20106 (91.37%, 97.02%, and 89.00%) were inhibited at the corresponding concentrations (10, 30, and 50\u2009mg\/mL) in RAW264.7 cells stimulated by lipopolysaccharide. Moreover, ADRB2 and PTPN2 were the key target proteins associated with bleeding\u2010related and inflammatory\u2010related diseases, which were obtained from the results of network pharmacology analysis. WSEE also alleviated the expression of ADRB2 (50.53%, 56.11%, and 83.41%) and PTPN2 (95.87%, 96.56%, and 91.49%) in RAW264.7 cells stimulated by lipopolysaccharide, findings that align with the results of network pharmacology. These findings provided evidence for considering WSEE as a promising candidate for wound healing.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70726","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T04:38:52Z","timestamp":1754455132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Elucidation of the Hemostatic and Anti\u2010Inflammatory Effects of Walnut Shell Ethanol Extract by Network Pharmacology and Experimental Verification"],"prefix":"10.1002","volume":"13","author":[{"given":"Ying","family":"He","sequence":"first","affiliation":[{"name":"Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science Xi'an Jiao Tong University  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center 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Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science Xi'an Jiao Tong University  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science Xi'an Jiao Tong University  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeguo","family":"Feng","sequence":"additional","affiliation":[{"name":"Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science Xi'an Jiao Tong University  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, First Affiliated Hospital of Medical 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