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Various species of this genus have also been verified to possess strong anti\u2010diabetic activity. In this context, the current study was designed to investigate the antidiabetic potential to confirm the purported traditional use of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P.\u2009emodi<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Different solvent fractions of\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">P.\u2009emodi<\/jats:styled-content>\n                      ,\n                    <\/jats:italic>\n                    i.e., crude methanolic extract (Pe.Cr), n\u2010hexane (Pe.Hex), chloroform (Pe.Chf), ethyl acetate (Pe.EtAc), butanol (Pe.Bt) and aqueous (Pe.Aq) were used for in\u00a0vitro studies against \u03b1\u2010glucosidase, \u03b1\u2010 amylase, dipeptidyl peptidase\u20104 (DPP\u20104), and protein tyrosine phosphatase 1B (PTP\u20101B) using a spectrophotometer and microplate reader. Among all fractions, Pe.Bt had the most prominent activity and was subjected to GC\u2013MS analysis, in\u00a0vivo anti\u2010diabetic, pancreas protective, and hepatoprotective studies in alloxan\u2010induced diabetes rats. The animals administered with alloxan (except group1) having FBGL higher than 220\u2009mg\/dL were selected and placed in different groups. The first group, which served as a normal control, received normal saline. The second group received a 5% tween\u201080 suspension, which served as diabetic control. The 3rd, 4th and 5th groups were administered Pe.Bt at the doses of 150, 300, and 500\u2009mg\/kg body weight, respectively, through oral gavage. Group six received metformin 50\u2009mg\/kg and served as the standard group. Fasting Blood glucose level (FBGL) was checked for 21\u2009days. Liver function tests, insulin level, hepatoprotection, and pancreas protection study were investigated. The \u03b1\u2010glucosidase was inhibited effectively by Pe.Bt with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 626\u2009\u03bcg\/mL. The antioxidant activities also substantiated the excellent inhibitory potential of Pe.Bt. The IC\n                    <jats:sub>50<\/jats:sub>\n                    value of Pe.Bt against DPPH and ABTS was figured out to be 77 and 139\u2009\u03bcg\/mL, respectively, which was relatively comparable with ascorbic acid. Similarly, Pe.Bt was found active against PTP\u20101B and showed a moderate effect against DPP4 with IC\n                    <jats:sub>50<\/jats:sub>\n                    of 45.13 and 92.45, respectively. In the time frame of 21\u2009days, the metformin and Pe.Bt significantly decreased FBGL on the 21st day to 108 and 96\u2009mg\/dL, respectively. Similarly, rats treated with Pe.Bt (500\u2009mg\/kg) significantly lowered the level of alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and increased total protein level (TP) in comparison with the diabetic control group. The histopathological study revealed that rats treated with Pe.Bt (500\u2009mg\/kg) have a remarkable ameliorative effect on liver and pancreas histological architecture as compared to the diabetic control group. The findings of the current investigations indicated that Pe.Bt possesses strong anti\u2010diabetic, hepatoprotective, anti\u2010oxidant properties and alleviates alloxan\u2010induced pancreatic damage in diabetic rats.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70870","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T06:42:46Z","timestamp":1756795366000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Phytochemical Screening and Antidiabetic Potential of\n                    <i>Paeonia emodi<\/i>\n                    in Alloxan\u2010Induced Diabetic Rats: Investigative Study Toward Possible Mechanism"],"prefix":"10.1002","volume":"13","author":[{"given":"Jirui","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Pharmacy Heilongjiang Eye Hospital, Harbin  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4710-4504","authenticated-orcid":false,"given":"Muhammad","family":"Ibrar","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Bacha Khan University  Charsadda Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mir Azam","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Malakand  Chakdara Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3698-0616","authenticated-orcid":false,"family":"Abdullah","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Malakand  Chakdara Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Saeed Jan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Bacha Khan University  Charsadda Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeeha","family":"Shabnam","sequence":"additional","affiliation":[{"name":"Department of Chemistry Women University Mardan  Mardan KP Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maqsood ur","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Malakand  Chakdara Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1994.tb02249.x"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-0998-z"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-015-0010-2"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joad.2016.08.011"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12185952"},{"issue":"1","key":"e_1_2_11_7_1","first-page":"23","article-title":"Ethnomedicinal Survey of Plants of Valley Alladand Dehri, Tehsil Batkhela, District Malakand, Pakistan","volume":"3","author":"Alamgeer T. 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