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In\u00a0vitro, assessments were performed utilizing diverse antioxidant assays, along with evaluations of neuroprotective enzyme inhibition targeting acetylcholine and butyl choline enzymes, as well as antidiabetic activities against \u03b1\u2010amylase and \u03b1\u2010glucosidase and a potential candidate for a tyrosinase inhibitor. LC\u2010ESI\u2010QTOF\u2010MS identification provided a total of 70 compounds in the extracted samples of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. californica<\/jats:italic>\n                    <\/jats:styled-content>\n                    , including kaempferol 3\u2010(deoxyhexosyl\u2010hexoside)\u20107\u2010hexoside, rutin, quercetin dideoxyhexoside, caffeic acid hexoside, quinoline alkaloids, morphine derivatives, and scoulerine. Moreover, the ethanol extract exhibited the highest anti\u2010AChE (2.39\u2009mg GALAE\/g), while ethyl acetate exhibited anti\u2010BChE (3.31\u2009mg GALAE\/g), Ethanol\/Water (70%) anti\u2010tyrosinase (53.09\u2009mg KAE\/g) and anti\u2010glucosidase (1.09\u2009mmol ACAE\/g) activities. Additionally, the ethyl acetate extract effectively inhibited carbonic anhydrase I and II isoenzymes. Furthermore, the ethanol and ethanol\/water extracts demonstrated significant cytotoxicity against A549 lung cancer cells. However,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. californica<\/jats:italic>\n                    <\/jats:styled-content>\n                    partially\/weakly triggers the apoptosis of cancer cells. Furthermore, the investigation identified 885 target genes for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. californica<\/jats:italic>\n                    <\/jats:styled-content>\n                    's phytochemicals, 31 of which were familiar to insomnia. In silico studies demonstrated that protopine, rutin, eschscholtzidine, boldine, and (S)\u2010scoulerine exhibited notable inhibitory effects on insomnia\u2010related DRD5, DRD4, and SERT proteins. These findings highlight the potential pharmacological applications of the aerial parts of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. californica<\/jats:italic>\n                    <\/jats:styled-content>\n                    as a source for developing novel phytopharmaceuticals targeting various oxidative stress\u2010related conditions, including diabetes, cancer, Alzheimer's disease, and insomnia.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.70885","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T04:26:04Z","timestamp":1756873564000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comprehensive Metabolomic Profiling and Biological Activity Analysis of\n                    <scp>\n                      <i>Eschscholzia californica<\/i>\n                    <\/scp>\n                    Extracts Using\n                    <scp>LC<\/scp>\n                    \u2010\n                    <scp>ESI<\/scp>\n                    \u2010\n                    <scp>QTOF<\/scp>\n                    \u2010\n                    <scp>MS<\/scp>"],"prefix":"10.1002","volume":"13","author":[{"given":"Alina","family":"Kalyniukova","sequence":"first","affiliation":[{"name":"Faculty of Forestry and Wood Sciences Czech University of Life Sciences Prague  Suchdol Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shakeel","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Physiology and Biochemistry Laboratory, Department of Biology, Science Faculty Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunes","family":"Ak","sequence":"additional","affiliation":[{"name":"Physiology and Biochemistry Laboratory, Department of Biology, Science Faculty Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enver","family":"Saka","sequence":"additional","affiliation":[{"name":"Physiology and Biochemistry Laboratory, Department of Biology, Science Faculty Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tugce","family":"Duran","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics, Faculty of Medicine KTO Karatay University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurgul","family":"Abul","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Ataturk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"Ilhami","family":"Gulcin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Ataturk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Senkardes","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy Marmara University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Veysi","family":"Cetiz","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Harran University  Sanliurfa Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Physiology and Biochemistry Laboratory, Department of Biology, Science Faculty Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202300547"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.106000"},{"issue":"1","key":"e_1_2_6_4_1","first-page":"110","article-title":"A Review on \n                     Erodium cicutarium\n                  : A Potential Medicinal Plant","volume":"4","author":"Al\u2010Snafi A. 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