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This study employs response surface methodology (RSM) combined with single\u2010factor experiments to optimize the extraction conditions for ultrasound\u2010microwave associated extraction (UMAE) of MA. Based on the RSM, the optimal extraction process conditions for mulberry anthocyanins are as follows: solid\u2013liquid ratio of 1:20, ethanol concentration of 80%, and ultrasonic power of 340\u2009W. Under these conditions, the anthocyanin yield reached 5.98\u2009mg\/g. Biological activity, cell apoptosis, cell morphology, and cell invasion experiments all demonstrated that mulberry anthocyanins have an inhibitory effect on 4 T1 cells. These findings suggest that the extraction rate of anthocyanins from mulberry can be improved by UMAE, and it has also been demonstrated that mulberry anthocyanins possess notable antitumor activity, highlighting their potential for therapeutic applications. Antioxidant assays revealed that MA obtained via UMAE exhibited significantly stronger scavenging capacities against DPPH\u00b7, \u00b7OH, and ABTS\u00b7 radicals compared to conventional solvent extraction. This enhanced activity may be attributed to milder extraction conditions, which better preserve MA's structural integrity and bioactive properties. Theoretically, these results corroborate that MA possesses remarkable antioxidant potential, consistent with its previously reported antitumor efficacy.<\/jats:p>","DOI":"10.1002\/fsn3.71022","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T07:30:01Z","timestamp":1759476601000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ultrasound\u2010Microwave Associated Extraction of Anthocyanins From Mulberry and Their Antioxidant and Anticancer Activities"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6200-3964","authenticated-orcid":false,"given":"Haibao","family":"Tang","sequence":"first","affiliation":[{"name":"School of Life Science Zhuhai College of Science and Technology  Zhuhai, Guangzhou China"},{"name":"School of Life Science Jilin University  Changchun, Jilin 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