{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:45:53Z","timestamp":1793681153924,"version":"4.5.1"},"reference-count":82,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>\n                      H\n                      <jats:styled-content style=\"fixed-case\">elminthostachys zeylanica<\/jats:styled-content>\n                    <\/jats:italic>\n                    (L.) hook (HZ), has recently gained attention as a potential herbal supplement for managing ulcerative colitis (UC) through its bioactive compounds. To comprehensively investigate HZ's therapeutic effects and underlying mechanisms on UC, we utilized network pharmacology and in\u00a0vitro and in\u00a0vivo analyses. The therapeutic potential of HZ was evaluated using a DSS\u2010induced mouse model of ulcerative colitis, alongside in\u00a0vitro cellular studies. A network pharmacology approach was first used to predict the active compounds and molecular targets of HZ. Subsequently, integrated experimental techniques\u2014including ELISA, Western blotting, histological analysis, immunofluorescence, flow cytometry, and molecular docking\u2014were employed to validate and support the predicted mechanisms. Network pharmacology analysis identified 15 active compounds in HZ, contributing to its multi\u2010target synergistic activity and anti\u2010inflammatory effects. HZ was found to modulate multiple inflammatory pathways, particularly the Toll\u2010like receptor 4 (TLR4) and NF\u2010\u03baB signaling pathways, regulating vital inflammatory mediators such as tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), interleukin\u20106 (IL\u20106), and interleukin\u20101\u03b2 (IL\u20101\u03b2), emphasizing its therapeutic potential in UC. ELISA, Western blot, and histological analyses confirmed that HZ significantly reduced colon inflammation. Immunofluorescence and flow cytometry analyses also demonstrated that HZ alleviated inflammation by regulating TLR4\/NF\u2010\u03baB and CD3 signaling pathways without involving apoptosis. Ultimately, molecular docking further identified core compounds, including Ugonin M, O, K, and R, which exhibited strong binding affinity to critical proteins in the TLR4\/NF\u2010\u03baB pathway, such as TAK1, IKK\u03b2, and RELA, underscoring their role in HZ's anti\u2010inflammatory mechanisms. Collectively, these findings provide a solid basis for further investigation into the mechanistic effects and broader clinical potential of HZ as a therapeutic approach for UC.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71139","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:53:50Z","timestamp":1761663230000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Combining Network Pharmacology, Molecular Docking, and Integrative Studies to Explore the Mechanism of\n                    <scp>\n                      <i>Helminthostachys zeylanica<\/i>\n                    <\/scp>\n                    in Alleviating Ulcerative Colitis"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5117-2791","authenticated-orcid":false,"given":"Chih\u2010Ting","family":"Lin","sequence":"first","affiliation":[{"name":"The School of Chinese Medicine for 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