{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T14:12:15Z","timestamp":1771423935476,"version":"3.50.1"},"reference-count":71,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The study evaluated the acaricidal potential of bioactive components of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Azadirachta indica<\/jats:italic>\n                    <\/jats:styled-content>\n                    against scabies mortality using both in\u00a0vitro and in silico approaches.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Sarcoptes scabiei<\/jats:italic>\n                    <\/jats:styled-content>\n                    were stimulated with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. indica<\/jats:italic>\n                    <\/jats:styled-content>\n                    at four concentrations (25\u2013100\u2009mg\/mL) at different intervals. The study assessed the cytotoxic, neuroactive, and detoxification\u2010modulating potential of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. indica<\/jats:italic>\n                    <\/jats:styled-content>\n                    , emphasizing their antibacterial, antioxidant, and enzyme\u2010inhibitory potential. LCMS was used for the characterization of phytoconstituents. In silico analysis encompassed target prediction, toxicity assessment, biological activity prediction, protein structure modeling, and gene expression analysis. Molecular docking assesses the binding affinities of bioactive components, and the ExPASy database predicts the physiochemical properties of glutathione transferase. In\u00a0vitro analysis suggests that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. indica<\/jats:italic>\n                    <\/jats:styled-content>\n                    has a dose\u2010dependent effect on\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. scabiei<\/jats:italic>\n                    <\/jats:styled-content>\n                    at different time intervals. It highlights the extract's multifaceted bioactivity with strong antioxidant activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20093.15\u2009mg\/mL) and potent antibacterial effects at higher concentrations. It exhibited mild to moderate hemolytic and significant AChE activity. Furthermore, it also showed GST inhibition, suggesting possible disruption of toxins. The binding affinity of 7\u2010desacetyl\u20107\u2010benzoylazadiradione showed significant inhibitory interaction with glutathione transferase (\u221212.324\u2009kcal\/mol). This phytoconstituent exhibited a high hyper\u2010geometric\n                    <jats:italic>p<\/jats:italic>\n                    value; the total interactions between transcription factors, kinases, and intermediate proteins are observed.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. indica<\/jats:italic>\n                    <\/jats:styled-content>\n                    serves as a natural substitute for managing mite infestations and offering insights into mechanisms by which its phytochemicals show inhibitory effects.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71204","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T14:21:37Z","timestamp":1764426097000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unlocking the Antioxidant, Enzyme Inhibitory and Acaricidal Potential of\n                    <scp>\n                      <i>Azadirachta indica<\/i>\n                    <\/scp>\n                    Phytoconstituents Using In\u00a0Vitro and In Silico Approaches"],"prefix":"10.1002","volume":"13","author":[{"given":"Tehreem","family":"Fatima","sequence":"first","affiliation":[{"name":"Department of Basic Sciences (Section Biochemistry) University of Veterinary and Animal Sciences  Lahore, Jhang Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mazhar","family":"Abbas","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences (Section Biochemistry) University of Veterinary and Animal Sciences  Lahore, Jhang Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kinza","family":"Zafar","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Railways Hospital, Islamic International Medical College  Rawalpindi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maha Gul","family":"Zafar","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences (Section Biochemistry) University of Veterinary and Animal Sciences  Lahore, Jhang Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Waqas","family":"Haider","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences (Section Biochemistry) University of Veterinary and Animal Sciences  Lahore, Jhang Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Haseeb","family":"Zafar","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics Railways Hospital, Islamic International Medical College  Rawalpindi Pakistan"},{"name":"Medical Unit 3 Lahore General Hospital  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5524-7735","authenticated-orcid":false,"given":"Muhammad","family":"Riaz","sequence":"additional","affiliation":[{"name":"Department of Allied Health Sciences University of Sargodha  Sargodha Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Munawar","family":"Iqbal","sequence":"additional","affiliation":[{"name":"School of Chemistry University of the Punjab  Lahore Pakistan"},{"name":"Renewable Energy and Environmental Technology Center Saudi Arabia University of Tabuk  Tabuk Saudi\u00a0Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew G.","family":"Mtewa","sequence":"additional","affiliation":[{"name":"Chemistry Section, Malawi Institute of Technology Malawi University of Science and Technology  Limbe Malawi"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065-018-0370-0"},{"issue":"2","key":"e_1_2_10_3_1","first-page":"250","article-title":"Antimicrobial Activities of Neem Extract (Azadirachta indica) Against Microbial Pathogens of Animal Origin","volume":"12","author":"Abd El\u2010Moez S. 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