{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:54:45Z","timestamp":1791482085733,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002713","name":"Imam Mohammed Ibn Saud Islamic University","doi-asserted-by":"publisher","award":["IMSIU\u2010DDRSP2501"],"award-info":[{"award-number":["IMSIU\u2010DDRSP2501"]}],"id":[{"id":"10.13039\/501100002713","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Stinging nettle (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Urtica dioica<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) remains under\u2010characterized despite its widespread ethnomedicinal use. Here, we combine quantitative phytochemistry, validated rodent pharmacology, and multi\u2010target molecular modeling to explore the analgesic and anti\u2010inflammatory potential of a Moroccan ecotype. HPLC\u2010PDA analysis of the hydro\u2010ethanolic extract (yield 14.8%) identified 15 phenolics, dominated by chlorogenic acid (26.5%), caffeic acid (18.8%), and p\u2010coumaric acid (12.8%). In\u00a0vivo, the polyphenolic fraction (100\u2009mg\/kg, p.o.) significantly reduced acetic\u2010acid writhing and carrageenan paw edema by 52% and 73%, respectively, and decreased both neurogenic and inflammatory phases of the formalin assay by over 59%. Similar efficacy was observed with the crude extract at 400\u2009mg\/kg, and acute oral toxicity tests showed no adverse effects up to 4\u2009g\/kg. Docking studies to mPGES\u20101, TRPV1, and BK2R revealed binding energies as low as \u221210.5\u2009kcal\/mol, highlighting rutin, hyperoside, and chlorogenic acid as promising lead compounds. ADME\/Tox profiling indicated high oral developability for apigenin and a broad safety margin for all leads. Overall, these findings position\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>U. dioica<\/jats:italic>\n                    <\/jats:styled-content>\n                    extracts as mechanistically validated phytopharmaceutical candidates for pain and inflammation management, supporting their potential for clinical development.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71253","type":"journal-article","created":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T09:11:46Z","timestamp":1764493906000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Urtica dioica<\/i>\n                    From El Menzel (Morocco): Phytochemical Analysis, In\u00a0Vivo and In Silico Evaluation of Analgesic and Anti\u2010Inflammatory Effects, and Toxicological Study With\n                    <scp>ADME<\/scp>\n                    Profiling"],"prefix":"10.1002","volume":"13","author":[{"given":"Sara","family":"Tlemcani","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology, Environment, Agri\u2010Food, and Health, Faculty of Sciences Dhar El Mahraz Sidi Mohamed Ben Abdellah University  Atlas Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Lahkimi","sequence":"additional","affiliation":[{"name":"Laboratory of Engineering, Molecular Organometallic Materials and Environment, Faculty of Sciences Dhar El Mahraz Sidi Mohamed Ben Abdellah University  Atlas Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oussama","family":"Khibech","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Chemistry and Environment (LCAE), Faculty of Science University Mohammed Premier  Oujda Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5442-6814","authenticated-orcid":false,"given":"Amal","family":"Elrherabi","sequence":"additional","affiliation":[{"name":"Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences University Mohammed 1st  Oujda Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9944-811X","authenticated-orcid":false,"given":"Mohamed","family":"Bouhrim","sequence":"additional","affiliation":[{"name":"Biological Engineering Laboratory, Faculty of Sciences and Techniques Sultan Moulay Slimane University  Beni Mellal Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahd A.","family":"Nasr","sequence":"additional","affiliation":[{"name":"Biology Department, College of Science Imam Mohammad Ibn Saud Islamic University (IMSIU)  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0810-4803","authenticated-orcid":false,"given":"Mohammed","family":"Al\u2010zharani","sequence":"additional","affiliation":[{"name":"Biology Department, College of Science Imam Mohammad Ibn Saud Islamic University (IMSIU)  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf Ahmed","family":"Qurtam","sequence":"additional","affiliation":[{"name":"Biology Department, College of Science Imam Mohammad Ibn Saud Islamic University (IMSIU)  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meryem","family":"Doubi","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Resources and Sustainable Development, Faculty of Sciences Ibn Tofail University \u2013 KENITRA \u2013 University Campus  Kenitra Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hicham","family":"Bekkari","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology, Environment, Agri\u2010Food, and Health, Faculty of Sciences Dhar El Mahraz Sidi Mohamed Ben Abdellah University  Atlas Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,30]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27248872"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2004.02.008"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2008.09.005"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae303"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2017.06.008"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(93)90098-A"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715760400016139"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2012.715172"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.29.2236"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2017.02.019"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.18311\/jnr\/2023\/29451"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology14060657"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2013.09.016"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17964"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3923\/rjmp.2015.1.13"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403106200"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.09.028"},{"issue":"2","key":"e_1_2_12_19_1","first-page":"193","article-title":"Antinociceptive and Anti\u2010Inflammatory Effects of Urtica dioica Leaf Extract in Animal Models","volume":"3","author":"Hajhashemi V.","year":"2013","journal-title":"Avicenna J. 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H.Nacer N.Ziani S.Lebrazi andN.Kertiou.2025.\u201cPrediction of Surface Tension of Propane Derivatives Using QSPR Approach and Artificial Neural Networks Prediction of Surface Tension of Propane Derivatives Using QSPR Approach and Artificial Neural Networks.\u201dhttps:\/\/doi.org\/10.48317\/IMIST.PRSM\/morjchem\u2010v13i2.54664."},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hermed.2012.11.001"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-015-0814-0"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-111-27849"}],"container-title":["Food Science &amp; Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.71253","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/fsn3.71253","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.71253","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T13:42:37Z","timestamp":1771422157000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/fsn3.71253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,30]]},"references-count":52,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/fsn3.71253"],"URL":"https:\/\/doi.org\/10.1002\/fsn3.71253","archive":["Portico"],"relation":{},"ISSN":["2048-7177","2048-7177"],"issn-type":[{"value":"2048-7177","type":"print"},{"value":"2048-7177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,30]]},"assertion":[{"value":"2025-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71253"}}