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In this study, we examined the potential effects of meranzin and its hydrate on glioblastoma (GBM) using artificial intelligence\u2010based in silico tools and in\u00a0vitro models. We used DIGEP\u2010Pred 2.0 to perform enrichment analysis of proteins affected by the two test compounds in GBM. ADMETlab 3.0 was applied to evaluate their medicinal chemistry properties and to predict absorption, distribution, metabolism, excretion, and toxicity (ADMET) profiles. We then selected the compound with higher pharmacological potential for in\u00a0vitro studies in the human GBM cell line. In addition, DIGEP\u2010Pred 2.0 was used to predict its effects on cancer stemness markers. Enrichment analysis suggested that both meranzin and its hydrate may have therapeutic potential for GBM. Based on the in silico comparisons from ADMETlab 3.0, meranzin hydrate was considered to have higher pharmacological potential. Meranzin hydrate exhibited cytotoxicity in U87 cells, but not in GBM8401 cells. In the U87 model, meranzin hydrate demonstrated anti\u2010invasion activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200933.12\u2009\u03bcM), although no inhibitory effect on colony formation was observed. Furthermore, meranzin hydrate suppressed the cancer stem cell property of sphere formation (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u200921.43\u2009\u03bcM). Moreover, in silico results showed that meranzin hydrate may downregulate the protein expression of two cancer stemness markers, ALDH1A1 and NANOG, which was further validated by in\u00a0vitro Western blot analysis. This study provides in silico assessments of the pharmacological potential, together with in\u00a0vitro evidence of the anti\u2010malignancy activity of the hydrate of meranzin in a human GBM cell line.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71332","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T08:57:13Z","timestamp":1766998633000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Artificial Intelligence\u2010Based In Silico Evaluation of the Pharmacological Potential and In\u00a0Vitro Anti\u2010Malignancy Effect in the Human Glioblastoma Cell Line of the Hydrate of the Coumarin Compound Meranzin Originating From the Edible Macroalga (\n                    <i>Bangia fuscopurpurea<\/i>\n                    )"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0370-1553","authenticated-orcid":false,"given":"Shi\u2010Ying","family":"Huang","sequence":"first","affiliation":[{"name":"College of Ocean Food and Biological Engineering Jimei University  Xiamen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Chen","family":"Chang","sequence":"additional","affiliation":[{"name":"Doctoral Degree Program in Marine Biotechnology National Sun Yat\u2010Sen University and Academia Sinica  Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7307-2468","authenticated-orcid":false,"given":"Charles Chien\u2010Chih","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Kaohsiung Medical University  Kaohsiung 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