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The objective of ENSSAIA was to investigate the nutrition, health, and food security conditions of indigenous peoples in Alagoas. This was a cross\u2010sectional, population\u2010based household survey assessing all 11 indigenous ethnic groups in the state, with a random selection of 14 out of 29 existing villages. All individuals registered in the Indigenous Health Care Subsystem of the selected villages were eligible for the study. A total of 1400 families were assessed, with data collected on environmental, demographic, and socioeconomic conditions; access to policies and public service infrastructure; household food security; health of children (&lt;\u20095\u2009years), pregnant women, women (19\u201359\u2009years), and the elderly (\u2265\u200960\u2009years); breastfeeding practices among children &lt;\u20092\u2009years; individuals with disabilities and genetic alterations; nutritional status through anthropometric indicators (all household members), biochemical markers: hemoglobin (children aged 6\u2009months to &lt;\u20095\u2009years and pregnant women), lipid profile, and glycated hemoglobin (women); dietary indicators (women, pregnant women, and children &lt;\u20095\u2009years); non\u2010communicable diseases; and alcohol and tobacco use (women, men, and elderly). The results produced by ENSSAIA are expected to support the formulation or revision of public policies that ensure greater access to healthcare, healthy food, and improved quality of life for indigenous peoples, considering their traditions, cultures, and ways of life.<\/jats:p>","DOI":"10.1002\/fsn3.71361","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T06:37:52Z","timestamp":1766731072000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Study of Nutrition, Health, and Food Security of Indigenous Peoples in the State of Alagoas, Brazil (ENSSAIA): Methodological Aspects"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5439-7910","authenticated-orcid":false,"given":"Tamara Rodrigues","family":"dos Santos","sequence":"first","affiliation":[{"name":"Faculty of Nutrition Federal University of Alagoas  Macei\u00f3 AL Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1889-8192","authenticated-orcid":false,"given":"Val\u00e9ria Clarisse","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Graduate Program in Public Health, School of Public Health University of S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0973-3908","authenticated-orcid":false,"given":"Marly Augusto","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Nutrition, School of Public Health University of S\u00e3o Paulo  S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2985-9890","authenticated-orcid":false,"given":"Monica Lopes","family":"de Assun\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Faculty of Nutrition Federal University of Alagoas  Macei\u00f3 AL Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5553-4431","authenticated-orcid":false,"given":"Andreia Herculano","family":"da Silva","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences and Health Federal University of Alagoas  Macei\u00f3 AL Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5804-5960","authenticated-orcid":false,"given":"Regina Coeli da Silva","family":"Vieira","sequence":"additional","affiliation":[{"name":"Institute of Health and Biotechnology Federal University of Amazonas  Coari AM Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1883-0734","authenticated-orcid":false,"given":"L\u00eddia Bezerra","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Faculty of Nutrition Federal University of Alagoas  Macei\u00f3 AL Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3275-1274","authenticated-orcid":false,"given":"Willams Henrique da Costa","family":"Maynart","sequence":"additional","affiliation":[{"name":"School of Nursing Federal University of Alagoas  Macei\u00f3 AL Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8453-017X","authenticated-orcid":false,"given":"Ewerton Amorim","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Federal University of Alagoas  Macei\u00f3 AL Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1789-3138","authenticated-orcid":false,"given":"Haroldo da Silva","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Faculty of Nutrition Federal University of Alagoas  Macei\u00f3 AL Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,25]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"978","article-title":"Standardization of DNA Extraction With NaCl From Oral Mucosa Cells: Application in PROP1 Gene Study","volume":"49","author":"Abr\u00e3o M. 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