{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:53:06Z","timestamp":1791003186526,"version":"4.1.0"},"reference-count":78,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diabetes mellitus (DM) is a metabolic disease characterized by persistent hyperglycemia, oxidative stress, and inflammation, often leading to severe complications. Current antidiabetic drugs exhibit efficacy; however, they are limited by adverse effects and diminishing long\u2010term benefits. Natural products, including\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Calotropis procera<\/jats:italic>\n                    <\/jats:styled-content>\n                    , are promising alternatives with minimal side effects: This study investigates the antidiabetic and protective effects of the methanolic extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. procera<\/jats:italic>\n                    <\/jats:styled-content>\n                    (CMECP) against diabetes and its complications using in\u00a0vitro and in\u00a0vivo models. in\u00a0vitro antioxidant, anti\u2010inflammatory, and hypoglycemic activities of the extract were conducted using established procedures while experimental rats were induced into diabetes with streptozotocin (STZ) and treated with distilled water, CMECP [150 and 300\u2009mg\/kg body weight (BW)] or 100\u2009mg\/kg b.wt metformin and assessed for their effects against diabetes and diabetes\u2010related complications. The extract exhibited dose\u2010dependent in\u00a0vitro antioxidant, anti\u2010inflammatory, and hypoglycemic activities. Treatment with the extract resulted in a 65% decrease in the fasting blood glucose levels of the diabetic rats at 300\u2009mg\/kg BW after the treatment period. The extract also improved insulin secretion, reversed dyslipidemia, ameliorated alterations in liver and kidney function biomarkers, reduced oxidative stress markers, and enhanced antioxidant defenses in STZ\u2010induced diabetic rats. Inflammatory biomarkers such as cyclooxyegase\u20102 and nuclear factor\u2010kappa B were suppressed, with improved hematological parameters and body weight. Additionally, CMECP mitigated diabetic\u2010induced neuronal cholinesterase activity without affecting neurotransmitter levels. The extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. procera<\/jats:italic>\n                    <\/jats:styled-content>\n                    demonstrated potent antidiabetic effects by modulating oxidative stress, inflammation, and biochemical dysfunctions in diabetes. These findings support its potential as a natural therapeutic agent for diabetes management. Further studies are ongoing to identify the constituents of the extract and elucidate their mechanisms of action for possible hit compounds identification for future antidiabetic drug development purposes.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71364","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T08:31:41Z","timestamp":1785918701000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the Antidiabetic Effects of\n                    <i>Calotropis procera<\/i>\n                    : Preclinical Assessment"],"prefix":"10.1002","volume":"14","author":[{"given":"Yu\u2010Cheng","family":"Kuo","sequence":"first","affiliation":[{"name":"Department of Pharmacology, College of Medicine Taipei Medical University  Taipei Taiwan"},{"name":"College of Osteopathic Medicine Sam Houston State University  Conroe 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Janeiro Brazil"},{"name":"Institute of Biomedical Sciences, Postgraduate Program in Pharmacology and Medicinal, Chemistry Federal University of Rio de Janeiro  Rio de Janeiro Brazil"},{"name":"Department of Chemical Sciences, Biochemistry Unit Afe\u2010Babalola University  Ado\u2010Ekiti Ekiti Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saheed","family":"Sabiu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Science, Faculty of Applied Sciences Durban University of Technology  Durban South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7832-8717","authenticated-orcid":false,"given":"Leandro","family":"Miranda\u2010Alves","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias da Sa\u00fade Instituto de Ci\u00eancias Biom\u00e9dicas Laborat\u00f3rio de Endocrinologia Experimental\u2014LEEx Universidade Federal do Rio de Janeiro  Rio de Janeiro 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