{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:02:28Z","timestamp":1789988548741,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402930"],"award-info":[{"award-number":["32402930"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007162","name":"Guangdong Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["2024A1515030170"],"award-info":[{"award-number":["2024A1515030170"]}],"id":[{"id":"10.13039\/501100007162","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010226","name":"Department of Education of Guangdong Province","doi-asserted-by":"publisher","award":["2024KTSCX208"],"award-info":[{"award-number":["2024KTSCX208"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science & Nutrition"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n This study characterizes the composition and blood components of ultramicro\u2010pulverized\n \n Polygonum chinense<\/jats:italic>\n <\/jats:styled-content>\n L<\/jats:italic>\n . and\n Atractylodes rhizome<\/jats:italic>\n powder (PAUP) and investigates its protective mechanisms against\n \n Escherichia coli<\/jats:italic>\n <\/jats:styled-content>\n (\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n )\u2010induced liver injury in broilers. PAUP constituents and serum metabolites were profiled by ultra\u2010high\u2010performance liquid chromatography\u2010mass spectrometry (UPLC\u2010MS). Fourteen\u2010day\u2010old broilers were randomly divided into 6 groups: control,\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n model, gentamicin, and PAUP high\/medium\/low\u2010dose groups. Broilers were infected with 10\n 10<\/jats:sup>\n \u2009CFU\/mL\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n O157:H7 via intraperitoneal injection. PAUP was administered 4\u2009h post\u2010\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n infection for 7\u2009days by gavage. Then, serum and liver samples from half of the chicks in each group were collected for further analysis. Recovery groups were analyzed after 7 additional days. UPLC\u2010MS identified 718 PAUP components, predominantly lipid, flavonoids, acids, and oxides. Serum metabolomics revealed 130 significantly elevated metabolites in PAUP groups, including atractylenolide I, linoleic acid, araliadiol, baicalin, gallocatechin, atractylodin, etc. PAUP normalized weight gain, reduced feed conversion ratio (FCR), and liver index in\n \n E. coli<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n infected broilers. It significantly decreased bacterial load, suppressed serum AST and TBA levels, and relieved liver histopathological damage. Furthermore, PAUP restored redox balance (increased GSH activity, decreased MDA and ROS levels) via Nrf2\u2010HO\u20101 pathway actvation (increased protein levels of Nrf2, Keap1, NQO1 and HO\u20101). PAUP obviously attenuated inflammation (decreased IL\u20106 and TNF\u2010\u03b1) through TLR4\/NF\u2010\u03baB inhibition. PAUP mitigates\n E. coli<\/jats:italic>\n \u2010induced liver injury through Nrf2\/HO\u20101\u2010mediated antioxidant activation and TLR4\/NF\u2010\u03baB inflammatory suppression.\n <\/jats:p>","DOI":"10.1002\/fsn3.71424","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T02:24:26Z","timestamp":1767666266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dual\u2010Pathway Defense: Ultramicro\u2010Pulverised Powder of\n Polygonum chinense<\/i>\n L. and\n Atractylodes rhizome<\/i>\n (PAUP) Rescues Broilers From\n E. coli<\/i>\n \u2010Triggered Liver Injury Through Modulation of Oxidative Stress and Inflammation"],"prefix":"10.1002","volume":"14","author":[{"given":"Jia\u2010Ci","family":"Cai","sequence":"first","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Na","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao\u2010Shan","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010Qiang","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi\u2010Peng","family":"Lv","sequence":"additional","affiliation":[{"name":"Xining Wildlife Park Xining Qinghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan\u2010Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Qin","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Xin","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Ming","family":"He","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6790-3480","authenticated-orcid":false,"given":"Lu\u2010Ping","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11060816"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.23602"},{"key":"e_1_2_12_4_1","first-page":"96","article-title":"Study on the Effects of Polygonum Chinense L. 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