{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:07:26Z","timestamp":1790690846697,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Due to the high incidence of periodontal diseases in pet cats and dogs, the purpose of this study is to screen bacteria with beneficial oral health effects and assess the effectiveness of its freeze\u2010dried cell. In this study, we explored the effects of oral probiotics by determining the bacteriostatic ability, percentages of self\u2010aggregation and co\u2010aggregation in\u00a0vitro, and through microbial community analysis, the alleviating effect of freeze\u2010dried cells on periodontitis and its mechanism of action were evaluated.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lactobacillus zeae<\/jats:italic>\n                      N165\n                    <\/jats:styled-content>\n                    significantly inhibited\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and it had high co\u2010aggregation rates of 96.78% and 88.94% with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>F. nucleatum<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , respectively. In\u00a0vivo, freeze\u2010dried\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. zeae<\/jats:italic>\n                      N165\n                    <\/jats:styled-content>\n                    cells significantly reduced alveolar bone resorption,\n                    <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\n                    \u2010\u03b1, and\n                    <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                    \u20106 levels in rats with periodontitis, maintained a healthy oral and intestinal microbial community structure, and regulated the dominant species to alleviate periodontitis. The prediction of functions by the\n                    <jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content>\n                    database analysis of oral flora revealed that freeze\u2010dried\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. zeae<\/jats:italic>\n                      N165\n                    <\/jats:styled-content>\n                    cells may alleviate periodontitis via four pathways: reduction of glutamine degradation, modulation of lipopolysaccharide levels, biosynthesis of polyketide glycan units, and biotin metabolism.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. zeae<\/jats:italic>\n                      N165\n                    <\/jats:styled-content>\n                    showed promising results in both in\u00a0vitro and in\u00a0vivo experiments, suggesting new directions for the oral probiotics industry.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71493","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T07:42:13Z","timestamp":1770018133000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Oral Administration of Freeze\u2010Dried\n                    <scp>\n                      <i>Lactobacillus zeae<\/i>\n                    <\/scp>\n                    Alleviates Periodontitis by Affecting the Oral and Intestinal Flora"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6970-7377","authenticated-orcid":false,"given":"Qimeng","family":"Liang","sequence":"first","affiliation":[{"name":"Food Science and Engineering College, Beijing University of Agriculture Beijing Laboratory of Food Quality and Safety  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0514-268X","authenticated-orcid":false,"given":"Zixin","family":"Kang","sequence":"additional","affiliation":[{"name":"Food Science and Engineering College, Beijing University of Agriculture Beijing Laboratory of Food Quality and Safety  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Song","sequence":"additional","affiliation":[{"name":"Inner Mongolia Mengniu Dairy (Group) Co. 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