{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:07:30Z","timestamp":1790690850940,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260638"],"award-info":[{"award-number":["82260638"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Panax notoginseng<\/jats:italic>\n                    saponins (PNS), the primary bioactive constituents derived from the traditional herb\n                    <jats:italic>Panax notoginseng<\/jats:italic>\n                    , have been used in nutritional supplements. However, their molecular mechanisms against hyperlipidemia\u2010induced liver injury remain to be fully elucidated. In this study, supplementation with PNS (200\u2009mg\/kg diet) for 12\u2009weeks significantly ameliorated high\u2010fat diet (HFD)\u2010induced liver injury in C57BL\/6J mice, as evidenced by improved serum lipid profiles and attenuated hepatic oxidative stress and inflammation. Lipidomics analysis indicated that PNS administration restored hepatic lipid homeostasis, particularly by normalizing dysregulated triglycerides. Mechanistically, PNS markedly suppressed the activation of key inflammatory signaling pathways, including both the TLR4\/MyD88\u2010mediated NF\u2010\u03baB and MAPK (ERK, JNK, p38) cascades. Complementary in\u00a0vitro experiments in LPS\u2010stimulated HepG2 cells confirmed that PNS attenuated cellular injury and downregulated these pathways. Notably, the protective effect of PNS was not additive to that of the specific TLR4 inhibitor TAK\u2010242, indicating a functional convergence on the TLR4 signaling axis. Collectively, these findings demonstrate that PNS protects against HFD\u2010induced liver injury by improving lipid metabolism and attenuating hepatic inflammation via mechanisms involving the suppression of TLR4\u2010mediated NF\u2010\u03baB and MAPK signaling, providing a refined mechanistic basis for its application as a dietary supplement in metabolic liver health.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71544","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T13:54:56Z","timestamp":1770990896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Panax notoginseng<\/i>\n                    Saponins Ameliorate High\u2010Fat Diet\u2010Induced Liver Injury via Mechanisms Involving\n                    <scp>TLR4<\/scp>\n                    \u2010Mediated Signaling and Lipid 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