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However, the effects of PBL water extract (PBLE) on alcohol\u2010induced liver disease remain unexplored. This study investigated the hepatoprotective effects of PBLE using a chronic\u2010plus\u2010single\u2010binge ethanol feeding model. PBLE (100 or 200\u2009mg\/kg\/day) was orally administered in combination with an ethanol diet. Mice were euthanised 9\u2009h post\u2010binge, and serum and liver tissues were collected for histological, biochemical, and molecular analyses. Six components (adenine, adenosine, hypoxanthine, inosine, benzoic acid, and uridine) were isolated from PBLE by ultra\u2010high\u2010performance liquid chromatography. PBLE treatment alleviated hepatic morphological changes, such as lipid droplet accumulation and hepatocytic ballooning, and reduced the elevated liver enzymes and triglyceride levels in the serum. Moreover, PBLE attenuated against the altered expression of alcohol metabolism\u2010related enzymes (alcohol dehydrogenase, aldehyde dehydrogenase, and cytochrome P450 2E1) in the liver. PBLE also exhibited anti\u2010inflammatory and antioxidant effects by normalizing the hepatic expression of p65, inducible nitric oxide synthase, cyclooxygenase\u20102, interleukin\u20101 beta, and tumor necrosis factor alpha, as well as nuclear factor erythroid 2\u2010related factor 2, haem oxygenase 1, glutathione peroxidase 3, superoxide dismutase, and catalase. In conclusion, PBLE may exert therapeutic effects on alcohol\u2010induced liver injury by improving alcohol metabolism and reducing oxidative stress and inflammation. These findings indicate that the edible beetle PBLs may serve as a hepatoprotective functional food ingredient.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71616","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:45:31Z","timestamp":1772988331000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Effect of\n                    <i>Protaetia brevitarsis<\/i>\n                    Larvae Extract on Alcoholic Liver Disease in Mice"],"prefix":"10.1002","volume":"14","author":[{"given":"Sueun","family":"Lee","sequence":"first","affiliation":[{"name":"Herbal Medicine Resources Research Center Korea Institute of Oriental Medicine  Naju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Hye","family":"Seo","sequence":"additional","affiliation":[{"name":"Herbal Medicine Resources Research Center Korea Institute of Oriental Medicine  Naju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Soo","family":"Seo","sequence":"additional","affiliation":[{"name":"Herbal Medicine Resources Research Center Korea Institute of Oriental Medicine  Naju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeon\u2010Hwa","family":"Nam","sequence":"additional","affiliation":[{"name":"Herbal Medicine Resources Research Center Korea Institute of Oriental Medicine  Naju Republic of Korea"},{"name":"Department of Crop Science &amp; Biotechnology Jeonbuk National University  Jeonju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Lee","sequence":"additional","affiliation":[{"name":"Herbal Medicine Resources Research Center Korea Institute of Oriental Medicine  Naju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2180-4860","authenticated-orcid":false,"given":"Joong\u2010Sun","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and BK21 Plus Project Team Chonnam National University  Gwangju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3236-7416","authenticated-orcid":false,"given":"Ji Hye","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Korean Medicine Pusan National University  Yangsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,6]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000090173"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2000.20189"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2011.134"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.032"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-017-6159-8_1"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2011.01660.x"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.12.013"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1363131"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/life10070116"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931\u2010015\u20100264\u20109"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530\u20100277.2008.00828.x"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.21336"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fufo.2023.100219"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.09.044"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(89)90338-9"},{"key":"e_1_2_13_17_1","doi-asserted-by":"crossref","unstructured":"Esser M. 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