{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:10:55Z","timestamp":1789168255189,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007713","name":"Bahauddin Zakariya University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007713","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Carissa carandas<\/jats:styled-content>\n                      \u2014\n                    <\/jats:italic>\n                    a member of the family Apocynaceae, has gained tremendous attention from the scientific community for its impressive nutritional and health\u2010promoting features. There is little information available on the toxicological risks associated with consuming the fruit; therefore, the present study aimed to investigate the toxicity of the commercially viable doses of the fruit and its extracts in an experimental rat model. A 28\u2010day sub\u2010acute feeding trial was performed on male albino rats using 5%\u201310% supplementation of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. carandas<\/jats:italic>\n                    <\/jats:styled-content>\n                    dehydrated powder and methanol fruit extracts. Our results demonstrated a significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.05) increase in hemoglobin and hematocrit concentration, from 11 to 14\u2009g\/dL and 35%\u201346%, respectively. Hematological results further confirmed improved hematopoiesis and thrombocytopoiesis due to dispensing\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. carandas<\/jats:italic>\n                    <\/jats:styled-content>\n                    and its methanol extract, anticipating a significant increase in platelet counts from 627 to 1090\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    \/\u03bcL. The study's hepatic and renal histopathological findings also demonstrated no visible degenerative or inflammatory risks on sub\u2010acute administered doses of fruit powder and extracts. The results indicate that lower doses of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. carandas<\/jats:italic>\n                    <\/jats:styled-content>\n                    powder and extracts, that is, &lt;\u200910% dietary supplementation, do not elicit any detrimental or toxicological effect in the rats, suggesting\n                    <jats:italic>C carandas<\/jats:italic>\n                    to be a valuable and promising candidate for developing value\u2010added functional foods to alleviate the risks of nutritional and other adverse health conditions.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71647","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:42:04Z","timestamp":1773405724000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>\n                      <i>Carissa carandas<\/i>\n                    <\/scp>\n                    L. and Its Methanol Extracts Promote Hematopoiesis and Thrombocytopoiesis: A Sub\u2010Acute Toxicological Study in Experimental Rats"],"prefix":"10.1002","volume":"14","author":[{"given":"Wisha","family":"Saeed","sequence":"first","affiliation":[{"name":"Department of Food Science &amp; Technology, Faculty of Food Science &amp; Nutrition Bahauddin Zakariya University (60800)  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Qamar","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology, Faculty of Food Science &amp; Nutrition Bahauddin Zakariya University (60800)  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8660-6040","authenticated-orcid":false,"given":"Raheel","family":"Suleman","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology, Faculty of Food Science &amp; Nutrition Bahauddin Zakariya University (60800)  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-0060","authenticated-orcid":false,"given":"Rai Muhammad","family":"Amir","sequence":"additional","affiliation":[{"name":"Institute of Food Science &amp; Nutrition Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0951-0982","authenticated-orcid":false,"given":"Muhammad","family":"Waseem","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, Faculty of Agriculture and Environment Islamia University of Bahawalpur  Bahawalpur Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9269-8172","authenticated-orcid":false,"given":"Sheraz Ahmed","family":"Bhatti","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Faculty of Veterinary Sciences Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asher Abdur","family":"Rehman","sequence":"additional","affiliation":[{"name":"Higher School of Medicine, Faculty of Medicine and Health Care Al\u2010Farabi Kazakh National University  Almaty Republic of Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqra","family":"Akram","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology, Faculty of Food Science &amp; Nutrition Bahauddin Zakariya University (60800)  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Zulqarnain","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology, Faculty of Food Science &amp; Nutrition Bahauddin Zakariya University (60800)  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3218-3684","authenticated-orcid":false,"given":"Robert","family":"Mugabi","sequence":"additional","affiliation":[{"name":"Department of Food Technology and Nutrition Makerere University  Kampala Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Food Science &amp; Technology, Faculty of Food Science &amp; Nutrition Bahauddin Zakariya University (60800)  Multan Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/jbcpp\u20102019\u20100042"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1108\/NFS\u201009\u20102017\u20100201"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.22038\/ajp.2018.11615"},{"key":"e_1_2_12_5_1","first-page":"2397","volume-title":"AIP Conference Proceedings","author":"Aichayawanich S.","year":"2021"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/512369"},{"key":"e_1_2_12_7_1","first-page":"14","article-title":"Carissa carandas Linn. 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