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The prebiotic function in\u00a0vitro and the anti\u2010constipation efficacy of garlic polysaccharides (GP) in loperamide\u2010induced constipated mice were evaluated. During in\u00a0vitro simulated digestion and fecal fermentation, GP showed digestive resistance properties and enhanced abundance of the probiotic bacteria\n                    <jats:italic>Bifidobacterium<\/jats:italic>\n                    and\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    . In\u00a0vivo, GP significantly attenuated intestinal oxidative stress. Moreover, GP treatment dose\u2010dependently ameliorated constipation parameters. Notably, GP restored colonic barrier integrity: GP potently upregulated the mRNA expression of tight junction proteins ZO\u20101 and occludin in the mechanical barrier and exhibited the most substantial restoration of both MUC2 and MUC4 expression levels in the mucins barrier, as well as reduced pro\u2010inflammatory cytokines of TNF\u2010\u03b1, IL\u20101\u03b2, IL\u201017 and elevated anti\u2010inflammatory IL\u201010 in the immune barrier. Furthermore, GP regulated VIP\u2010cAMP\u2010PKA\u2010AQP3 signaling pathway, which subsequently promoted intestinal water transport and mucus secretion. GP significantly regulated the gut microbiota\u2010SCFAs axis by lowering the\n                    <jats:italic>Firmicutes\/Bacteroidetes<\/jats:italic>\n                    ratio and suppressing the level of pathobiont\n                    <jats:italic>Desulfovibrio<\/jats:italic>\n                    . GP intervention significantly elevated total SCFAs compared to the MOD group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). These results elucidated GP's multi\u2010target mechanism against functional constipation via antioxidant, barrier\u2010repairing, VIP pathway\u2010regulating, and microbiota\u2010directed SCFAs axis.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71659","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:11:30Z","timestamp":1773810690000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Anti\u2010Constipation Effect of Garlic Polysaccharides: Roles of Gut Barrier Integrity,\n                    <scp>VIP<\/scp>\n                    Pathway, and the Microbiota\u2010\n                    <scp>SCFAs<\/scp>\n                    Axis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7884-5810","authenticated-orcid":false,"given":"Jingfang","family":"Li","sequence":"first","affiliation":[{"name":"College of Life Sciences and Food 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Engineering, Hebei University of Engineering  Handan China"},{"name":"Department of Nutrition and Health China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9098-3332","authenticated-orcid":false,"given":"Shimin","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Food Engineering, Hebei University of Engineering  Handan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Chai","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Food Engineering, Hebei University of Engineering  Handan 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