{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:06:46Z","timestamp":1790626006233,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007162","name":"Guangdong Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["2024A1515030170"],"award-info":[{"award-number":["2024A1515030170"]}],"id":[{"id":"10.13039\/501100007162","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402930"],"award-info":[{"award-number":["32402930"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010226","name":"Department of Education of Guangdong Province","doi-asserted-by":"publisher","award":["2024KTSCX208"],"award-info":[{"award-number":["2024KTSCX208"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Heat stress (HS) impairs liver function and negatively affects the growth performance of poultry. This study evaluates the protective effects and underlying mechanisms of Yinhuang Oral Liquid (YOL) in alleviating liver injury in heat\u2010stressed broilers, highlighting its potential application in mitigating HS\u2010related liver damage. Broilers were randomly allocated into six groups: Control (23\u00b0C\u2009\u00b1\u20092\u00b0C), Model (HS, 35\u00b0C\u2009\u00b1\u20092\u00b0C for 8\u2009h\/day for 7 consecutive days), Positive (HS\u2009+\u2009Vitamin C, 60\u2009mg\/kg), YOL\u2010H (HS\u2009+\u2009YOL, 10\u2009g\/kg), YOL\u2010M (HS\u2009+\u2009YOL, 5\u2009g\/kg), and YOL\u2010L (HS\u2009+\u2009YOL, 2.5\u2009g\/kg). Vitamin C and YOL were administered intragastrically 1\u2009h after HS exposure for 7\u2009days. HS impaired growth performance, as evidenced by decreased final body weight, average daily feed intake (ADFI), and average daily gain (ADG), along with an increased feed conversion ratio (FCR). YOL supplementation increased ADG compared with the model group. Histopathological analysis showed severe inflammatory cell infiltration around the hepatic central vein under heat stress, which was markedly attenuated by vitamin C and YOL. YOL activated the hepatic NRF2\u2013KEAP1 signaling pathway by increasing the p\u2010P62\/P62 ratio, increasing NRF2 and its downstream antioxidant proteins HO\u20101 and NQO1, and downregulating KEAP1 expression. Network pharmacology analysis identified Sirt1 as a crucial bridging target linking YOL to heat stress. Molecular docking further demonstrated that the top four compounds\u2014baicalin, wogonoside, oroxylin A\u20107\u2010O\u2010glucuronide, and acacetin\u2010exhibited high binding affinity with the Sirt1 receptor. YOL significantly upregulated Sirt1, GPX4, and SLC7A11 protein levels and restored mitochondrial cristae integrity while promoting autolysosome formation. It also restored HS\u2010induced suppression of PINK1 and Parkin. In conclusion, YOL effectively mitigates HS\u2010induced oxidative stress in broilers by activating Sirt1, which coordinately enhances the NRF2\u2013KEAP1 and PINK1\/Parkin signaling pathways.<\/jats:p>","DOI":"10.1002\/fsn3.71660","type":"journal-article","created":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T04:34:17Z","timestamp":1773635657000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ying\u2010Huang Oral Liquid Ameliorates Oxidative Stress in the Liver of Heat\u2010Stressed Broilers via\n                    <scp>KEAP1<\/scp>\n                    \u2010\n                    <scp>NRF2<\/scp>\n                    and\n                    <scp>PINK1<\/scp>\n                    \/Parkin Pathway"],"prefix":"10.1002","volume":"14","author":[{"given":"Zi\u2010Hao","family":"Li","sequence":"first","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Ci","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Lin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Li","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Ming","family":"He","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6790-3480","authenticated-orcid":false,"given":"Lu\u2010Ping","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12172297"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12080733"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey243"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00099\u201020"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020\u2010023\u201000714\u20106"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2022.102157"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13061007"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13496"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.114073"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203163"},{"issue":"9","key":"e_1_2_12_12_1","first-page":"78","article-title":"Study on Safety Pharmacology of Yinhuang Oral Liquid","volume":"56","author":"Li Z. 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