{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:07:11Z","timestamp":1790172431365,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Liver toxicity is a major health concern caused by pharmaceutical exposure, poisons like CCl4, or environmental contaminants. The CCl4\u2010induced liver toxicity model is extensively used to study hepatic damage, such as oxidative stress and fibrosis. In current study, synergistic effect of natural compounds Lycopene (Lyc) and L\u2010Carnitine (L\u2010Car) possessing the antioxidant activity was assessed to mitigate the liver fibrosis induced by CCl4 in male rat model. CCl4 treated rats showed significant decrease in body weight (21.62%\u2009\u00b1\u20090.83%) alongside elevated liver enzymes ALP (276\u2009\u00b1\u20096.62), AST (283\u2009\u00b1\u20094.53), ALT (138\u2009\u00b1\u20090.74), bilirubin (1.73\u2009\u00b1\u20090.74) and lactate dehydrogenase (LDH) an injury marker (0.778%\u2009\u00b1\u20090.06%). After treatment of CCl4 induced fibrosis with Lyc\u2009+\u2009L\u2010Car, significantly increased body weight of rats was observed (34.39%\u2009\u00b1\u20090.77%). Liver enzymes also showed remarkable improvement after treatment with Lyc\u2009+\u2009L\u2010Car (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.001). Combined Lyc\u2009+\u2009L\u2010Car group showed reduced the LDH level (0.246%\u2009\u00b1\u20090.02%), fibrosis gene markers TIMP\u20101 and Col1\u03b11 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009\u2264\u20090.001) and increased antioxidant enzyme activity of SOD (0.56\u2009\u00b1\u20090.04\u2009U\/dL) and CAT (0.489\u2009\u00b1\u20090.004\u2009U\/dL). Histological analysis showed a marked improvement in liver architecture, with reduced fibrosis appearance. These findings suggest that combination of Lyc and L\u2010Car supplementation effectively counteracts fibrosis, oxidative stress, and liver enzymes elevation, supporting its potential role as a dietary therapeutic agent for metabolic and hepatic disorders. Future recommendations include conducting long\u2010 term clinical trials in humans to validate these findings, exploring optimal dosages for dietary lycopene supplementation, and investigating its molecular mechanisms of action.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71689","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T06:10:27Z","timestamp":1775887827000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ameliorative Effects of Lycopene and L\u2010Carnitine on\n                    <scp>CCl<\/scp>\n                    <sub>4<\/sub>\n                    \u2010Induced Liver Fibrosis Rat Model"],"prefix":"10.1002","volume":"14","author":[{"given":"Adeel","family":"Shahid","sequence":"first","affiliation":[{"name":"University Institute of Medical Laboratory Technology The University of Lahore  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2721-9238","authenticated-orcid":false,"given":"Somia","family":"Shehzadi","sequence":"additional","affiliation":[{"name":"University Institute of Medical Laboratory Technology The University of Lahore  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moazzam","family":"Salamat","sequence":"additional","affiliation":[{"name":"University Institute of Medical Laboratory Technology The University of Lahore  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Shafiq","sequence":"additional","affiliation":[{"name":"University Institute of Medical Laboratory Technology The University of Lahore  Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6582-6051","authenticated-orcid":false,"given":"Zaid Talal Abdulqader","family":"Ali","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Saba Region  Marib Yemen"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/HC9.0000000000000679"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201200182"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111831"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2023.12.023"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2021.114480"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11122318"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1320062"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9121310"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-23276-9"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.97"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.3791\/60695"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8RA08204A"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/JCMA.0000000000000699"},{"issue":"7","key":"e_1_2_14_15_1","first-page":"634","article-title":"TIMP\u20101 Promotes Expression of MCP\u20101 and Macrophage Migration by Inducing Fli\u20101 in Experimental Liver Fibrosis","volume":"12","author":"Huang X.","year":"2024","journal-title":"Journal of Clinical and Translational Hepatology"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.121131"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13050548"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2020.613225"},{"issue":"2","key":"e_1_2_14_19_1","first-page":"184","article-title":"L\u2010Carnitine Ameliorates the Liver by Regulating Alpha\u2010SMA, iNOS, HSP90, HIF\u20101alpha, and RIP1 Expressions of CCL4\u2010Toxic Rats","volume":"24","author":"Karabulut D.","year":"2021","journal-title":"Iranian Journal of Basic Medical Sciences"},{"issue":"8","key":"e_1_2_14_20_1","first-page":"1889","article-title":"Genetic Deficiency of Nuclear Receptor ROR\u03b1 in Myeloid Cell Exacerbates Lipid Dysregulation and Chronic Subretinal Inflammation in Aging Mice","volume":"66","author":"Kushwah N.","year":"2025","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.893"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i16.2912"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12986-021-00592-x"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.3325\/cmj.2023.64.243"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.12.005"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2019.09.002"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.21608\/blj.2025.381090.1070"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S186726"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2022.5157"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2023.1214734"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2021.e08488"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpp\/rgaf053"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.70549"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3662"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/jpm12071053"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cimb47110914"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxrep.2025.101976"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.653037"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2023.2287930"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2024.2328521"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-019-2641-2"},{"issue":"4","key":"e_1_2_14_42_1","first-page":"1177","article-title":"Effect of Lycopene Supplementation on Lipid Profile, Blood Glucose and Electrolyte Homeostasis in Thioacetamide Induced Liver Cirrhosis","volume":"36","author":"Zaidi S. N. F.","year":"2023","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2024.08.020"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.937484"}],"container-title":["Food Science &amp; Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.71689","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/fsn3.71689","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.71689","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:38:01Z","timestamp":1790170681000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/fsn3.71689"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/fsn3.71689"],"URL":"https:\/\/doi.org\/10.1002\/fsn3.71689","archive":["Portico"],"relation":{},"ISSN":["2048-7177","2048-7177"],"issn-type":[{"value":"2048-7177","type":"print"},{"value":"2048-7177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71689"}}