{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:07:19Z","timestamp":1790172439298,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2024JJ9527"],"award-info":[{"award-number":["2024JJ9527"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ischemic stroke induces neuroinflammation that exacerbates neuronal damage. The Chinese Medicine Xuming Zhusan (XMZS) has shown promising efficacy in stroke treatment, but its mechanisms are unclear. This study aimed to explore the effect of XMZS on microglial\u2010mediated neuroinflammation and explore its molecular targets. In\u00a0vivo experiments were conducted using a mouse model of cerebral ischemia\u2013reperfusion injury induced by middle cerebral artery occlusion (MCAO), and in\u00a0vitro studies were performed on primary microglia and cortical neurons with Oxygen\u2013Glucose Deprivation\/Reoxygenation (OGD\/R) models. Behavioral assessments, histological analyses, Western blotting, co\u2010immunoprecipitation, confocal microscopy, and enzyme\u2010linked immunosorbent assay (ELISA) were employed to evaluate the neuroprotective effects of XMZS and its regulatory role in the TLR4\/MYD88\/NF\u2010\u03baB pathway. Results showed that high\u2010dose (63\u2009g\/kg) XMZS improved neurological and cognitive functions, reduced infarct volume and brain edema in MCAO mice. Mechanistically, XMZS suppressed microglial activation, decreased the levels of pro\u2010inflammatory cytokines (IL\u20101\u03b2, IL\u20106, TNF\u2010\u03b1), and downregulated the expression of TLR4, MYD88, and phosphorylated p65 in the TLR4\/MYD88\/NF\u2010\u03baB pathway. Additionally, cinditioned medium from XMZS\u2010treated microglia enhanced the viability of primary neurons. In conclusion, XMZS exerts neuroprotective effects against cerebral ischemia\u2013reperfusion injury by modulating the TLR4\/MYD88\/NF\u2010\u03baB pathway, thereby attenuating microglia\u2010mediated neuroinflammation.<\/jats:p>","DOI":"10.1002\/fsn3.71734","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T05:06:16Z","timestamp":1776402376000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Xuming Zhusan Decoction Attenuates Post\u2010Stroke via Modulating\n                    <scp>TLR4<\/scp>\n                    \/\n                    <scp>MYD88<\/scp>\n                    \/\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    Pathway in Mice"],"prefix":"10.1002","volume":"14","author":[{"given":"Xi","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Changsha Hospital of Traditional Chinese Medicine (The Eighth Hospital of Changsha)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Changsha Hospital of Traditional Chinese Medicine (The Eighth Hospital of Changsha)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Changsha Hospital of Traditional Chinese Medicine (The Eighth Hospital of Changsha)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi'an","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Changsha Hospital of Traditional Chinese Medicine (The Eighth Hospital of Changsha)  Changsha Hunan 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Traditional Chinese Medicine (The Eighth Hospital of Changsha)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Changsha Hospital of Traditional Chinese Medicine (The Eighth Hospital of Changsha)  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6949-3567","authenticated-orcid":false,"given":"Zhijun","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Changsha Hospital of Traditional Chinese Medicine (The Eighth Hospital of Changsha)  Changsha Hunan 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S.Gong X.Chen et\u00a0al.2020.\u201cAnalgecine Inhibits NF\u2010kB to Regulate Microglia Polarization by TLR4 MyD88\u2010Dependent and MyD88\u2010Independent Pathways in Ischemic Stroke.\u201d Research Square.","DOI":"10.21203\/rs.3.rs-29490\/v1"}],"container-title":["Food Science &amp; Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.71734","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/fsn3.71734","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.71734","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:39:12Z","timestamp":1790170752000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/fsn3.71734"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/fsn3.71734"],"URL":"https:\/\/doi.org\/10.1002\/fsn3.71734","archive":["Portico"],"relation":{},"ISSN":["2048-7177","2048-7177"],"issn-type":[{"value":"2048-7177","type":"print"},{"value":"2048-7177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71734"}}