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In this study, an ultrasound\u2010assisted enzymatic extraction was used to prepare a\n                    <jats:italic>Ganoderma lingzhi<\/jats:italic>\n                    triterpenoid\u2010enriched extract (GLE), and its hepatoprotective effects were evaluated through physicochemical characterization, network pharmacology, and animal experiments. The optimized extraction yielded 16.32\u2009\u00b1\u20090.22\u2009mg of total triterpenoids per gram of dry material. Functional screening identified GLE70 as the most active fraction, showing strong antioxidant activity, alcohol dehydrogenase activation, inhibition of carbohydrate\u2010hydrolysing enzymes, and bile acid binding capacity. HPLC\u2010TOF\u2010MS\/MS analysis showed that GLE70 contained multiple triterpenoids, with eight relatively abundant components including ganoderic acids B and D. Network pharmacology and molecular docking predicted\n                    <jats:italic>AKT1<\/jats:italic>\n                    ,\n                    <jats:italic>TNF<\/jats:italic>\n                    , and\n                    <jats:italic>SRC<\/jats:italic>\n                    as key targets associated with the hepatoprotective effects of GLE70. In\u00a0vivo, GLE70 increased AKT1 expression, reduced TNF\u2010\u03b1 and SRC\u2010associated inflammatory signals, and improved hepatic function, antioxidant status, and lipid metabolism, suggesting a coordinated multi\u2010target regulatory effect. Overall, this study obtained a bioactive triterpenoid\u2010enriched fraction from\n                    <jats:italic>G. lingzhi<\/jats:italic>\n                    and provided insights into its multi\u2010target regulatory characteristics in alleviating ALD, providing a basis for further application of\n                    <jats:italic>G. lingzhi<\/jats:italic>\n                    triterpenoids in liver protection.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71893","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:01:30Z","timestamp":1778821290000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Triterpenoid\u2010Enriched\n                    <scp>GLE70<\/scp>\n                    Fraction From\n                    <i>Ganoderma lingzhi<\/i>\n                    Ameliorates Alcoholic Liver Disease via Multi\u2010Target 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