{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:12:22Z","timestamp":1789992742412,"version":"4.0.1"},"reference-count":66,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T00:00:00Z","timestamp":1779494400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A study of the phytochemical composition, as well as in\u00a0vitro and in\u00a0vivo neuropharmacological activities of methanol extract of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. indicum<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:italic>MECI<\/jats:italic>\n                    ) was carried out along with an in silico prediction. GC\u2013MS\/MS and FTIR results showed that the PEs contained various bioactive components like flavonoids, terpenoids, alkaloids, heterocyclic substances. In mice,\n                    <jats:italic>MECI<\/jats:italic>\n                    (200 and 400\u2009mg\/kg, i.p.) caused significant dose\u2010dependent anxiolytic effects as indicated by the elevated plus maze test (increased duration and entries in open arms; 166.6\u2009\u00b1\u200914.71\u2009s, ***\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), hole\u2010board test (enhanced head\u2010dipping frequency; 53.4\u2009\u00b1\u20094.71; ***\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), light\/dark box test (greatest latency time without suppression of locomotion). Antidepressant\u2010like effect was evidenced by decreased immobility in forced swim (48.5%\u201355.1% reduction) and tail suspension test (83.0\u2009\u00b1\u20092.60 to 72.4\u2009\u00b1\u20092.33\u2009s), being at the level of efficacy of fluoxetine (67.4\u2009\u00b1\u20092.87\u2009s). The extract did not have a sedative effect as measured by the open matter and hole\u2010cross tests. ADME profiling suggested well oral absorption and BBB permeability and PASS prediction indicated possible neuropharmacological activity. Molecular docking revealed that the major constituents (methyl 10,13\u2010dimethyltetradecanoate; \u22128.5\u2009kcal\/mol) and tetrazolo[1,5\u2010b]pyridazine (\u22128.1\u2009kcal\/mol) showed strong binding scores with CNS targets as compared to reference drugs. Molecular dynamics simulation revealed that Tetrazolo[1,5\u2010b]pyridazine forms a structurally stable complex with the 2Z5X protein, supporting its potential anxiolytic activity through favorable conformational stabilization. Toxicity predictions suggested low hepatotoxicity and cardiotoxicity. These results in turn offer mechanistic explanation for ethnomedicinal use of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. indicum<\/jats:italic>\n                    <\/jats:styled-content>\n                    to manage anxiety and depression and thus endorse it as a multi\u2010target natural therapeutic agent against the two mood disorders.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.71918","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T08:01:51Z","timestamp":1779523311000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phytochemical Characterization and Neuropharmacological Assessment of\n                    <i>Combretum indicum<\/i>\n                    Methanolic Extract With Integrated Molecular Docking and Dynamics Simulation for Anxiety and Depression Therapy"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5157-9298","authenticated-orcid":false,"given":"Zobayed","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Science University of Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Ashraful","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Jashore University of Science and Technology  Jashore Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakib Ahamed","family":"Bhuiyan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Science University of Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabikun","family":"Naher","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Science University of Chittagong  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4058-8449","authenticated-orcid":false,"given":"Nilufar","family":"Sultana","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Manarat International University  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4511-8697","authenticated-orcid":false,"given":"S. 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Naim","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Biological Science University of Chittagong  Chattogram 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