{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T21:20:07Z","timestamp":1787606407334,"version":"build-2736575974"},"reference-count":68,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100010724","name":"Amasya \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010724","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Galanthus elwesii<\/jats:italic>\n                    <\/jats:styled-content>\n                    is a pharmaceutically valuable Amaryllidaceae species known for its diverse bioactive metabolites; however, the relationship between its phytochemical profile, antioxidant capacity, and in\u00a0vivo biological effects remains poorly understood. In this study, aerial (APE) and belowground (BPE) parts were extracted using water, methanol, and ethanol, and evaluated through a combined in\u00a0vitro and in\u00a0vivo approach. Total phenolic and flavonoid contents were quantified, and antioxidant activities were assessed via DPPH radical\u2010scavenging and Fe\n                    <jats:sup>2+<\/jats:sup>\n                    metal\u2010chelation assays, including IC\n                    <jats:sub>50<\/jats:sub>\n                    determination. The in\u00a0vivo effects of selected extracts were investigated in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    <\/jats:styled-content>\n                    through larval toxicity and developmental survival assays, adult lifespan analysis, and oxidative stress biomarkers (TAS, TOS, and OSI). Phytochemical composition and antioxidant activity exhibited strong organ\u2010 and solvent\u2010dependent variability, with belowground extracts generally demonstrating higher phenolic content and stronger radical\u2010scavenging and metal\u2010chelating capacities. In\u00a0vivo, most extracts showed low\u2010to\u2010moderate developmental toxicity at selected concentrations, while certain belowground extracts significantly extended lifespan at subtoxic doses. Oxidative status measurements revealed reduced TOS and OSI levels alongside maintained or increased TAS, indicating effective suppression of oxidative stress rather than direct antioxidant action. Correlation analysis revealed moderate negative correlations between phenolic\/flavonoid content and antioxidant activity, although these relationships did not reach statistical significance. Overall, these findings suggest that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>G.\u2009elwesii<\/jats:italic>\n                    <\/jats:styled-content>\n                    extracts exert organ\u2010dependent phytochemical and bioactivity profiles and may act as in\u00a0vivo redox modulators associated with improvements in oxidative balance and lifespan\u2010related parameters.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.72055","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T15:38:43Z","timestamp":1782401923000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Organ\u2010Dependent Antioxidant, Redox\u2010Modulating, and Lifespan Effects of\n                    <scp>\n                      <i>Galanthus elwesii<\/i>\n                    <\/scp>\n                    Extracts in\n                    <scp>\n                      <i>Drosophila melanogaster<\/i>\n                    <\/scp>"],"prefix":"10.1002","volume":"14","author":[{"given":"Osman","family":"Ertan","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Institute of Science Amasya University  Amasya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0473-4653","authenticated-orcid":false,"given":"Arif","family":"Ayar","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques, Sabuncuo\u011flu \u015eerefeddin Health Services Vocational School Amasya University  Amasya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9210-6907","authenticated-orcid":false,"given":"Ebru Bat\u0131","family":"Ay","sequence":"additional","affiliation":[{"name":"Department of Plant and Animal Production, Suluova Vocational School Amasya University  Amasya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.70225"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-025-05093-5"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-08409-4"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00128-010-0159-x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.ip34"},{"issue":"2","key":"e_1_2_10_7_1","first-page":"297","article-title":"Comperative Study of Maceration and Ultrasonic\u2010Assisted Extraction of Galantamine and Lycorine Content and Antioxidant Activity of \n                     Galanthus elwesii\n                   Hook","volume":"9","author":"Ay E. B.","year":"2020","journal-title":"Academic Journal of Agriculture"},{"issue":"3","key":"e_1_2_10_8_1","first-page":"690","article-title":"Effect of Phosphorus Fertilization on Phenolic Compounds and Antioxidant Activity in \n                     Galanthus elwesii\n                   Hook","volume":"7","author":"Ay E. B.","year":"2023","journal-title":"International Journal of Agriculture Environment and Food Sciences"},{"issue":"2","key":"e_1_2_10_9_1","first-page":"376","article-title":"Anthelmintic Activities of Methanol Extracts of Five Different Geophyte Plant Species","volume":"25","author":"Ayd\u0131n \u00c7.","year":"2022","journal-title":"Kahramanmara\u015f S\u00fct\u00e7\u00fc \u0130mam University Journal of Agriculture and Nature"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/63.1.35"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-018-2292-8"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.200900380"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202513698"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26125694"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects15110870"},{"issue":"2","key":"e_1_2_10_16_1","first-page":"66","article-title":"Effects of Herbal Safflower Oil on Longevity and Oxidative Stress","volume":"8","author":"Co\u015fkun R.","year":"2024","journal-title":"Commagene Journal of Biology"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000101510"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00093a019"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21667"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.70086"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2003.11.015"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2005.08.008"},{"issue":"3","key":"e_1_2_10_23_1","first-page":"1","article-title":"Investigation of Effect of Probiotic Use on Organism Behaviours in \n                     Drosophila melanogaster","volume":"4","author":"Fidan M.","year":"2022","journal-title":"Sabuncuoglu Serefeddin Health Sciences"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/35041687"},{"issue":"1","key":"e_1_2_10_25_1","article-title":"Geophytes of East Anatolia (Turkey)","volume":"5","author":"F\u0131rat M.","year":"2015","journal-title":"Manas Journal of Agriculture Veterinary and Life Sciences"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants13243577"},{"key":"e_1_2_10_27_1","first-page":"1","article-title":"A Comparative Study of Nutrient Composition, Proteolytic Activity, Phytochemical Profiles, Vitamin C Content, and Antioxidant Properties in the Peels of Selected Perennial Fruits","volume":"14","author":"Gopalraaj J.","year":"2024","journal-title":"Agricultural Research"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.115.183392"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA14666B"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2004.02.012"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajme.2017.09.001"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43088-024-00469-x"},{"issue":"1","key":"e_1_2_10_33_1","first-page":"107","article-title":"Quantitative Determination of Galanthamine and Lycorine in \n                     Galanthus elwesii\n                   by HPLC\u2010DAD","volume":"11","author":"Kaya G. I.","year":"2014","journal-title":"Turkish Journal of Pharmaceutical Sciences"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.2147\/CIA.S158513"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1527-3458.2002.tb00221.x"},{"key":"e_1_2_10_36_1","article-title":"Measurement of Lifespan in \n                     Drosophila melanogaster","volume":"71","author":"Linford N. J.","year":"2013","journal-title":"Journal of Visualized Experiments: JoVE"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2019.100982"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/59.1.B3"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2005.12.009"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00051"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.117943"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB07.613"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e41605"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.70714"},{"key":"e_1_2_10_45_1","first-page":"195","article-title":"Mediterranean Flora and Its Conservation in Turkey: With Special Reference to Monocot Geophytes","volume":"23","author":"\u00d6zhatay N.","year":"2013","journal-title":"Flora Mediterranea"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.23798"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.08.070"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-6371-3_18"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2015.04.040"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8416763"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2023.0247"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.107145"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2007.03.002"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.169101"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3308"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-025-04921-9"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2021.12.267"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00694"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tma.2024.09.001"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0230-3"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.5344\/ajev.1977.28.1.49"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13070787"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.023762"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336934.2024.2420453"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.70268"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41514-024-00155-3"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13040394"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jafr.2025.102138"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2024.103402"}],"container-title":["Food Science &amp; Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.72055","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/fsn3.72055","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.72055","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T20:33:47Z","timestamp":1787603627000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/fsn3.72055"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":68,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/fsn3.72055"],"URL":"https:\/\/doi.org\/10.1002\/fsn3.72055","archive":["Portico"],"relation":{},"ISSN":["2048-7177","2048-7177"],"issn-type":[{"value":"2048-7177","type":"print"},{"value":"2048-7177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2026-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e72055"}}