{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:02:04Z","timestamp":1790172124931,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Piper chaba<\/jats:italic>\n                    , a traditionally important spice and medicinal plant, was investigated for its anticancer and antimicrobial potential using crude root extracts and root\u2010derived synthesized silver\/silver chloride nanoparticles. Cytotoxicity assessment using the brine shrimp nauplii assay revealed LC\n                    <jats:sub>50<\/jats:sub>\n                    values of 2445.56, 1983.56, and 1306.98\u2009\u03bcg\/mL for the aqueous PcRE (Aq), ethanolic PcRE (Et) extracts, and nanoparticle formulations PcRE (Np), respectively, indicating enhanced bioactivity of the nanoparticles. Antibacterial evaluation showed that PcRE (Np) exhibited lower MBC\/MIC ratios (1.60\u20132.64) compared to PcRE (Aq) (2.00\u20132.13) and PcRE (Et) (2.17\u20132.20), suggesting superior bactericidal efficacy. At 250\u2009\u03bcg\/mL, PcRE (Np) demonstrated the highest antibiofilm activity against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (ATCC 27853) (74%), followed by PcRE (Aq) (67%) and PcRE (Et) (42%). In\u00a0vivo anticancer studies using Swiss albino mice bearing Ehrlich ascites carcinoma (EAC) showed tumor growth inhibition of 26.3%, 32.0%, and 41.5% for PcRE (Aq), PcRE (Et), and PcRE (Np), respectively, at a dose of 2.5\u2009mg\/kg\/day. Consistently, MTT assays against EAC cells indicated higher growth inhibition by PcRE (Np) (12.7%) compared with PcRE (Et) (7.5%) and PcRE (Aq) (6.65%) at 300\u2009\u03bcg\/mL. Treatment with PcRE (Np) significantly prolonged survival and improved hematological parameters, while also modulating cancer\u2010associated blood glucose levels. Histopathological analysis revealed notable hepatic tissue recovery in PcRE (Np)\u2010treated groups, exceeding that observed with fluorouracil. Molecular docking studies further supported these findings, demonstrating strong interactions of major\n                    <jats:italic>P. chaba<\/jats:italic>\n                    phytochemicals with COX\u20102 and p53 proteins. Collectively, these results highlight PcRE (Np) as a promising antimicrobial and anticancer candidate.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.72087","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T07:53:13Z","timestamp":1783410793000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Docking, Antitumor and Antimicrobial Activities of Extracts and Synthesized Silver Nanoparticles From\n                    <i>Piper chaba<\/i>\n                    Roots"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2239-9625","authenticated-orcid":false,"given":"Sultana","family":"Rajia","sequence":"first","affiliation":[{"name":"Department of Nutrition and Food Engineering Varendra University  Rajshahi Bangladesh"},{"name":"Center for Interdisciplinary Research Varendra University  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Ariful","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Genetic Engineering and Biotechnology University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Aminul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alima","family":"Khanam","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iffat Ara","family":"Tasnim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Varendra University  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Shamim","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Varendra University  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imtiaj","family":"Hasan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Rajshahi  Rajshahi Bangladesh"},{"name":"Department of Microbiology University of Rajshahi  Rajshahi Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-023-04279-z"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e38709"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/biol-2020-0094"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1119550"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2022.104001"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2021.e07228"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2012.12.003"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.33073\/pjm-2011-005"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ddc3010011"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.17348\/era.4.0.223-231"},{"key":"e_1_2_12_12_1","first-page":"593","article-title":"Observation of Self\u2010Prepared Medicine in Treating Gastric Diseases","volume":"15","author":"Chen T.","year":"2008","journal-title":"Journal of Practical Medical Technology"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.05.020"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-60208-1"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2011.08.009"},{"key":"e_1_2_12_16_1","first-page":"151","article-title":"Growth Inhibition of Human Colon Cancer Cells by Plant Compounds","volume":"21","author":"Duessel S.","year":"2008","journal-title":"Clinical Laboratory Science"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2024.2438689"},{"key":"e_1_2_12_18_1","first-page":"17030","article-title":"Antibiofilm Activity of \n                     Piper longum\n                   Ethanol Extract Against Methicillin\u2010Resistant \n                     Staphylococcus aureus\n                   Strains","volume":"7","author":"Everlyne I. 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