{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:01:53Z","timestamp":1790172113094,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated whether\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lactobacillus acidophilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    supplementation provides additional benefits to a whey protein\u2010based high\u2010protein diet (HPD) in diet\u2010induced obese rats. After obesity induction, 48 male Wistar rats were assigned to four 12\u2010week interventions (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912\/group): ND1 (140\u2009g\/kg whey protein isolate), ND2 (ND1\u2009+\u2009\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. acidophilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), HPD1 (500\u2009g\/kg whey protein isolate) and HPD2 (HPD1\u2009+\u2009\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. acidophilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). Supplemented groups received 5\u2009\u00d7\u200910\n                    <jats:sup>8<\/jats:sup>\n                    \u2009CFU\/rat\/day by oral gavage. Food intake, body\u2010weight change, regional fat mass, serum biochemical markers and gut microbiota composition were evaluated. HPD\u2010fed rats showed lower food intake, sustained body\u2010weight loss and reduced visceral and epididymal fat masses compared with rats receiving normal\u2010protein diets. Body\u2010weight trajectories differed between dietary regimens (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.013), whereas weight loss did not differ significantly between HPD1 and HPD2 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.06). Fasting glycemia remained unchanged. At month 3, ND1 showed the highest triglyceride and total cholesterol concentrations, while ND2, HPD1 and HPD2 displayed lower terminal values. High\u2010protein feeding was associated with higher terminal urea and uric acid concentrations, whereas creatinine did not progressively increase. In the microbiota subset, HPD2 was characterized by higher relative abundances of\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    and\n                    <jats:italic>Turicibacter<\/jats:italic>\n                    . The whey protein\u2010based HPD was the principal factor associated with body\u2010weight reduction, lower adiposity and favorable lipid responses in obese rats. Under high\u2010protein conditions,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. acidophilus<\/jats:italic>\n                    <\/jats:styled-content>\n                    did not confer an additional benefit for body weight or serum lipids, although its effect on gut microbiota composition warrants further functional investigation.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.72118","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T04:39:31Z","timestamp":1784781571000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High\u2010Protein Diet Combined With\n                    <i>Lactobacillus acidophilus<\/i>\n                    Improves Weight Loss, Lipid Metabolism, and Gut Microbiota in Obese Rats"],"prefix":"10.1002","volume":"14","author":[{"given":"Aicha","family":"Sbaihia","sequence":"first","affiliation":[{"name":"Laboratory of Bioeconomics, Food Safety and Health, Faculty of Natural Sciences and Life Abdelhamid Ibn Badis University of Mostaganem  Mostaganem Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6167-6260","authenticated-orcid":false,"given":"Bouasria","family":"Benbouziane","sequence":"additional","affiliation":[{"name":"Laboratory of Bioeconomics, Food Safety and Health, Faculty of Natural Sciences and Life Abdelhamid Ibn Badis University of Mostaganem  Mostaganem Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1505-6184","authenticated-orcid":false,"given":"Muhammad","family":"Waqar","sequence":"additional","affiliation":[{"name":"Food Technology and Innovation Research Center of Excellence, School of Agricultural Technology and Food Industry Walailak University  Nakhon si thammarat Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4708-4307","authenticated-orcid":false,"given":"Mohamed Cherif","family":"Bentahar","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Physiology, Faculty of Natural Sciences and Life Abdelhamid Ibn Badis University of Mostaganem  Mostaganem Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4857-9467","authenticated-orcid":false,"given":"Djilali","family":"Benabdelmoumene","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Physiology, Faculty of Natural Sciences and Life Abdelhamid Ibn Badis University of Mostaganem  Mostaganem Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7865-3093","authenticated-orcid":false,"given":"Said","family":"Dahmouni","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Animal Physiology, Faculty of Natural Sciences and Life Abdelhamid Ibn Badis University of Mostaganem  Mostaganem Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumia","family":"Keddari","sequence":"additional","affiliation":[{"name":"Laboratory of Bioeconomics, Food Safety and Health, Faculty of Natural Sciences and Life Abdelhamid Ibn Badis University of Mostaganem  Mostaganem Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6097-471X","authenticated-orcid":false,"given":"Wasim S. 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