{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:53:12Z","timestamp":1791003192963,"version":"4.1.0"},"reference-count":79,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Vitis vinifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaves are abundant by\u2010products of the grape industry, often discarded as agricultural waste. This study explores the in\u00a0vitro and in silico antioxidant, antidiabetic, and antibacterial properties of hydromethanolic crude extracts from grapevine leaves from five local grapevine varieties in Algeria (Chikki, Azorith\u2010fereinguel, Bezoul El\u2010khadem d'Alg\u00e9rie, Amesski El Hamma, and Hasram). Extracts were prepared via ultrasonic\u2010assisted extraction, with subsequent phytochemical profiling performed by liquid chromatography\/mass spectrometry (LC\/MS). The analysis revealed 17 identified phenolic compounds, primarily flavanols, and a few phenolic acids, including Isoquercitrin, Hesperidin, and Rutin, which were major compounds. Critically, the Azorith\u2010fereinguel variety exhibited superior antioxidant and antidiabetic activity (IC50: 3.93\u2009\u03bcg\/mL for DPPH; 43.61\u2009\u03bcg\/mL for ABTS; 75.12\u2009\u03bcg\/mL for RP; 22.46\u2009\u03bcg\/mL for \u03b1\u2010amylase; and 25.30\u2009\u03bcg\/mL for \u03b1\u2010glucosidase), outperforming many standard natural extracts and approaching the efficacy of synthetic controls. Furthermore, three varieties (Azorith\u2010fereinguel, Bezoul El\u2010khadem, Amesski El Hamma) showed potent antibacterial activity against four pathogens, with minimum inhibitory concentrations lower than standard antibiotics, indicating a potential solution for antibiotic resistance. In silico analyses revealed that hesperidin and rutin have high binding affinities to key enzyme active sites, providing a mechanistic basis for the observed bioactivity. These findings transform grapevine leaves from agricultural waste into a high\u2010value, multi\u2010functional natural product. This work provides a scientific foundation for developing grapevine leaf extracts into affordable, accessible nutraceuticals, functional food ingredients, or natural food preservatives for managing oxidative stress, diabetes, and bacterial infections, while simultaneously valorizing an abundant agro\u2010industrial by\u2010product within a circular food economy, particularly in resource\u2010limited settings where grape by\u2010products are abundant.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.72214","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T06:01:26Z","timestamp":1786428086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["New Insights Into the Phytochemical Composition and Biological Potential of Leaf Extracts From Algerian Autochthonous\n                    <scp>\n                      <i>Vitis vinifera<\/i>\n                    <\/scp>\n                    L."],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2951-645X","authenticated-orcid":false,"given":"Radhia","family":"Belmanaa","sequence":"first","affiliation":[{"name":"Laboratory of Genetics, Biotechnology and Valorization of Bio\u2010Resources University of Biskra  Biskra Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4157-1523","authenticated-orcid":false,"given":"Azzeddine","family":"Zeraib","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Biotechnology and Valorization of Bio\u2010Resources University of Biskra  Biskra Algeria"},{"name":"Biotechnology, Water, Environment and Health Laboratory, Faculty of Natural and Life Sciences University of Abbes Laghrour  Khenchela Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziane","family":"Laiadi","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Biotechnology and Valorization of Bio\u2010Resources University of Biskra  Biskra Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Simozrag","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Biotechnology and Valorization of Bio\u2010Resources University of Biskra  Biskra Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2785-4949","authenticated-orcid":false,"given":"Khadra Afaf","family":"Bendrihem","sequence":"additional","affiliation":[{"name":"Biotechnology, Water, Environment and Health Laboratory, Faculty of Natural and Life Sciences University of Abbes Laghrour  Khenchela Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1914-973X","authenticated-orcid":false,"given":"Ayomide Victor","family":"Atoki","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Kampala International University  Ishaka Uganda"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3536-6358","authenticated-orcid":false,"given":"Mohammed","family":"Messaoudi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Higher Normal School of Kouba (ENS) Laboratory of Research on Bioactive Products and Biomass Valorization  Algiers Algeria"},{"name":"Department of Chemistry, Higher Normal School of Kouba (ENS) Laboratory N\u2014Body and Structure of Matter  Algiers 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