{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:49:51Z","timestamp":1792511391925,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chuju (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Dendranthema morifolium<\/jats:italic>\n                    <\/jats:styled-content>\n                    (Ramat.) Tzvel. cv.), a traditional Chinese medicinal herb with both medicinal and edible properties. It is widely used in tea beverages and functional foods due to its rich flavonoid and polysaccharide content. Our previous studies demonstrated that the total flavonoids of Chuju (TFCJ) alleviate cerebral ischemia\u2013reperfusion injury (CIRI) in rats. However, the potential metabolic pathways regulated by TFCJ remain unclear, and its therapeutic targets require further investigation. This study aims to elucidate the neuroprotective mechanisms of TFCJ. LC\u2013MS analysis identified 42 compounds within TFCJ. Animal experiments showed that TFCJ significantly reduced cerebral infarct volume, elevated SOD and GSH\u2010Px levels, and decreased MDA levels in the brain. Non\u2010targeted metabolomic analysis suggested that TFCJ exerts its protective effects by modulating the tricarboxylic acid (TCA) cycle, glycolysis, pentose phosphate pathway (PPP), and aspartate\u2013glutamate metabolism. Pathway analysis revealed that the 4EBP1\/eIF4E signaling pathway may mediate the protective effects of TFCJ. In conclusion, this study analyzed the main components of TFCJ and provided evidence suggesting that the 4EBP1\/eIF4E signaling pathway might serve as a potential mediator of its neuroprotective effects against CIRI. These findings provide a theoretical basis for the application of TFCJ as both a functional food and a medicinal agent.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.72216","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T06:54:27Z","timestamp":1787900067000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of the Mechanism of Total Flavonoids From Chuju in Mitigating Cerebral Ischemia\u2013Reperfusion Injury in Rats Using Untargeted Metabolomics"],"prefix":"10.1002","volume":"14","author":[{"given":"Guanglei","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Traditional Chinese Medicine Bozhou University  Bozhou China"},{"name":"School of Food Science and Engineering Anhui Science and Technology University  Chuzhou 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University of Chinese Medicine  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianxi","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine Bozhou University  Bozhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyong","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine Bozhou University  Bozhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine Bozhou University  Bozhou 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