{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:59:13Z","timestamp":1790405953605,"version":"4.1.0"},"reference-count":114,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001798","name":"Edith Cowan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001798","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp;amp; Nutrition"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Cucurbitaceae<\/jats:italic>\n                    is a major plant family within the kingdom\n                    <jats:italic>Plantae<\/jats:italic>\n                    , encompassing popular food crops such as zucchini, squash, pumpkins, and melons. With the growing interest in discovering novel bioactive peptides (BPs) from various plant sources,\n                    <jats:italic>Cucurbitaceae<\/jats:italic>\n                    crops have emerged as a popular candidate for isolating nutrient\u2010enriched peptides due to their diverse applications in the nutritional and pharmaceutical sectors, with experimentally evidenced antihypertensive, antidiabetic, antimicrobial, and anticancer properties. However, current investigations are limited to a few economically important species and are seed\u2010centric within this family, with limited in\u00a0vivo and clinical validations for their in\u00a0vitro functional properties. In this review, we have summarized the peer\u2010reviewed original proteomic research and reviews, written in English, reported to date on peptide production from protein extraction to hydrolysis, identification, and functional characterization, as well as their industrial translation, excluding those without a standard functional validation. This work discusses and evaluates the applied protocols, limitations, and challenges in BP discovery and industrial utilization, highlighting understudied cucurbits as a potential source of novel BPs and insights into broadening future research scopes and providing opportunities aligned with plant\u2010derived peptide discovery.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.72338","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:54:50Z","timestamp":1789624490000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioactive Peptides From the\n                    <i>Cucurbitaceae<\/i>\n                    Family: From Identification and Health\u2010Promoting Functions to Industrial Utilization"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5868-1697","authenticated-orcid":false,"given":"Himani R.","family":"Kekiri Obada Gamage","sequence":"first","affiliation":[{"name":"School of Science Edith Cowan University  Joondalup Western Australia Australia"},{"name":"Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science Edith Cowan University  Joondalup Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahya","family":"Bahmani","sequence":"additional","affiliation":[{"name":"School of Science Edith Cowan University  Joondalup Western Australia Australia"},{"name":"CSIRO Agriculture and Food  Floreat Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utpal","family":"Bose","sequence":"additional","affiliation":[{"name":"School of Science Edith Cowan University  Joondalup Western Australia Australia"},{"name":"Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science Edith Cowan University  Joondalup Western Australia Australia"},{"name":"CSIRO Agriculture and Food  St. Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1560-3600","authenticated-orcid":false,"given":"Mekhala","family":"Vithana","sequence":"additional","affiliation":[{"name":"School of Science Edith Cowan University  Joondalup Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8463-805X","authenticated-orcid":false,"given":"Michelle L.","family":"Colgrave","sequence":"additional","affiliation":[{"name":"School of Science Edith Cowan University  Joondalup Western Australia Australia"},{"name":"Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science Edith Cowan University  Joondalup Western Australia Australia"},{"name":"CSIRO Agriculture and Food  St. Lucia Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4317-2027","authenticated-orcid":false,"given":"Ang\u00e9la","family":"Juh\u00e1sz","sequence":"additional","affiliation":[{"name":"School of Science Edith Cowan University  Joondalup Western Australia Australia"},{"name":"Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science Edith Cowan University  Joondalup Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016\u201011846"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2024.106343"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745\u20104514.2009.00239.x"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031445"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010018\u20102176\u2010y"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2022.11.024"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.22364\/eeb.14.23"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.4038\/cjs.v45i2.7387"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.17508\/cjfst.2018.10.2.16"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541\u20104337.12578"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.17795\/semj20586"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.2298\/jsc150615081b"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2015.06.054"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idairyj.2006.11.003"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10111738"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/np900174n"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/22311866.2019.1682044"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2012.07.028"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16015"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.904643"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods6050032"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2014.02.003"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8931"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-821389-6.00003-0"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33532"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/1177932220952739"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jafr.2022.100353"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.9734\/ejnfs\/2019\/v10i430117"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfutfo.2022.03.003"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092335"},{"key":"e_1_2_13_32_1","volume-title":"Faostat Statistical Database","author":"FAO","year":"2025"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2024.104529"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.07.187"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010021\u201000233\u20101"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cofs.2023.101039"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2014.12.019"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.66.9.3898\u20103904.2000"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2014.10.020"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2015.09.003"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2007.01.013"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.08.020"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2018.03.047"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2017.1282516"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2013.787626"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/np50018a002"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idairyj.2005.10.012"},{"key":"e_1_2_13_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations5030039"},{"key":"e_1_2_13_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.15163"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c00609"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217\u2010021\u201003783\u20101"},{"key":"e_1_2_13_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.4188"},{"key":"e_1_2_13_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541\u20104337.13050"},{"key":"e_1_2_13_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12161\u2010016\u20100732\u2010y"},{"key":"e_1_2_13_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27041343"},{"key":"e_1_2_13_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.01.062"},{"key":"e_1_2_13_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2019.03.007"},{"key":"e_1_2_13_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfpp.14443"},{"key":"e_1_2_13_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197\u2010018\u20103274\u2010z"},{"key":"e_1_2_13_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20103375\u20104_1"},{"key":"e_1_2_13_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20235978"},{"key":"e_1_2_13_62_1","unstructured":"Nag B. S.Medicherla andS. D.Sharma.2008.\u201cOrally Active Fraction ofMomordica charantia Active Peptides Thereof and Their Use in the Treatment of Diabetes.\u201d U.S. EP 1005274 B1."},{"key":"e_1_2_13_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cofs.2021.11.006"},{"key":"e_1_2_13_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jafr.2023.100861"},{"key":"e_1_2_13_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods8070245"},{"key":"e_1_2_13_66_1","doi-asserted-by":"publisher","DOI":"10.2141\/jpsa.0180018"},{"key":"e_1_2_13_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1977"},{"key":"e_1_2_13_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1958"},{"key":"e_1_2_13_69_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2017.1283516"},{"key":"e_1_2_13_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods9081018"},{"key":"e_1_2_13_71_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/2121878"},{"key":"e_1_2_13_72_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929866525666180809142326"},{"key":"e_1_2_13_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.08.018"},{"key":"e_1_2_13_74_1","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2014.980946"},{"key":"e_1_2_13_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2018.07.013"},{"key":"e_1_2_13_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2016.10.035"},{"key":"e_1_2_13_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2016.09.023"},{"key":"e_1_2_13_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2020.110788"},{"key":"e_1_2_13_79_1","doi-asserted-by":"publisher","DOI":"10.31357\/vjs.v26i01.6410"},{"key":"e_1_2_13_80_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205\u2010020\u201002565\u2010y"},{"key":"e_1_2_13_81_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods10040839"},{"key":"e_1_2_13_82_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24040-4_6"},{"key":"e_1_2_13_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2017.01.009"},{"key":"e_1_2_13_84_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20100610"},{"key":"e_1_2_13_85_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10091326"},{"key":"e_1_2_13_86_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/156060"},{"key":"e_1_2_13_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2004.03.002"},{"key":"e_1_2_13_88_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u20108\u2010280"},{"key":"e_1_2_13_89_1","doi-asserted-by":"publisher","DOI":"10.1080\/19476337.2014.1003199"},{"key":"e_1_2_13_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2016.10.049"},{"key":"e_1_2_13_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2021.01.004"},{"key":"e_1_2_13_92_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16024150"},{"key":"e_1_2_13_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-63505-1.00035-X"},{"key":"e_1_2_13_94_1","doi-asserted-by":"publisher","DOI":"10.1080\/19476337.2017.1411978"},{"key":"e_1_2_13_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.130494"},{"key":"e_1_2_13_96_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.975938"},{"key":"e_1_2_13_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2012.07.017"},{"key":"e_1_2_13_98_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00440"},{"key":"e_1_2_13_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2018.04.005"},{"key":"e_1_2_13_100_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c04678"},{"key":"e_1_2_13_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2017.12.062"},{"key":"e_1_2_13_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168\u20109452(98)00052\u20101"},{"key":"e_1_2_13_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.07.062"},{"key":"e_1_2_13_104_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3fo30347k"},{"key":"e_1_2_13_105_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0196\u20109781(03)00191\u20108"},{"key":"e_1_2_13_106_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068\u2010011\u20100122\u20106"},{"key":"e_1_2_13_107_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.127059"},{"key":"e_1_2_13_108_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.125165"},{"key":"e_1_2_13_109_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phyplu.2020.100016"},{"key":"e_1_2_13_110_1","volume-title":"Increasing Fruit and Vegetable Consumption to Reduce the Risk of Noncommunicable Diseases","author":"World Health Organization","year":"2023"},{"key":"e_1_2_13_111_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780813811048.ch3"},{"key":"e_1_2_13_112_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.29.1126"},{"key":"e_1_2_13_113_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.04.006"},{"key":"e_1_2_13_114_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.815640"},{"key":"e_1_2_13_115_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2014.09.008"}],"container-title":["Food Science &amp; Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.72338","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/fsn3.72338","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/fsn3.72338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:24:36Z","timestamp":1790403876000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/fsn3.72338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":114,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/fsn3.72338"],"URL":"https:\/\/doi.org\/10.1002\/fsn3.72338","archive":["Portico"],"relation":{},"ISSN":["2048-7177","2048-7177"],"issn-type":[{"value":"2048-7177","type":"print"},{"value":"2048-7177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-01-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e72338"}}