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This study demonstrated that 12\u2010week BSE supplementation (600\u2009mg\/kg diet) significantly ameliorated HFD\u2010induced renal dysfunction, reducing blood urea nitrogen (BUN) from 6.29 to 3.02\u2009mmol\/L (by 52.0%), uric acid (UA) from 56.38 to 17.25\u2009\u03bcmol\/L (by 69.4%), and serum creatinine (SCr) from 19.21 to 11.54\u2009\u03bcmol\/L (by 39.9%), while decreasing renal triglycerides (TG) from 3.86 to 2.43\u2009mmol\/g (by 37.1%) and total cholesterol (TC) from 19.47 to 14.43\u2009mmol\/g (by 25.9%). BSE also restored oxidative balance, increasing superoxide dismutase (SOD) activity from 4.37 to 6.54\u2009U\/mg protein (by 49.7%) and total antioxidant capacity (T\u2010AOC) from 1.78 to 2.97\u2009\u03bcmol\/mg protein (by 66.9%), with a reduction in malonaldehyde (MDA) from 1.68 to 1.19\u2009nmol\/mg protein (by 29.2%). Untargeted lipidomics revealed that BSE normalized four lipid mediators linked to lipotoxicity, including CAR 22:1, LPC 16:0, PI\u2010Cer 38:1; O3, and NAE 26:5. Mechanistically, BSE activated AMPK signaling (increasing p\u2010AMPK\u03b12 protein and\n                    <jats:italic>AMPK\u03b12<\/jats:italic>\n                    mRNA expression by 1.72\u2010fold and 2.13\u2010fold, respectively) to inhibit lipogenesis (downregulating SREBP1 and SCD by 21.4% and 37.3%, respectively) and promote fatty acid oxidation (upregulating SIRT1, PPAR\u03b1, CPT1A, and PGC1\u03b1 by 1.3\u2010, 2.1\u2010, 4.6\u2010, and 1.3\u2010fold, respectively), enhanced Nrf2\u2010driven antioxidant defense (upregulating\n                    <jats:italic>Nrf2<\/jats:italic>\n                    ,\n                    <jats:italic>HO\u20101<\/jats:italic>\n                    , and\n                    <jats:italic>NQO1<\/jats:italic>\n                    mRNA expression by 1.2\u2010, 3.3\u2010, and 1.9\u2010fold, respectively), and suppressed the NLRP3 inflammasome cascade (reducing NLRP3 by 29.0%, cleaved caspase\u20101 by 13.8%, and IL\u20101\u03b2 by 39.5%, respectively). These findings establish that BSE protects against HFD\u2010induced renal injury involving modulation of AMPK\/Nrf2 signaling and the NLRP3 inflammasome, supporting its potential as a dietary strategy for metabolic kidney disease.\n                  <\/jats:p>","DOI":"10.1002\/fsn3.72348","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:50:32Z","timestamp":1789995032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Broccoli Sprout Extract Restores Renal Lipid Metabolism in High\u2010Fat Diet\u2010Fed Mice Involving Modulation of\n                    <scp>AMPK<\/scp>\n                    \/Nrf2 Signaling and the\n                    <scp>NLRP3<\/scp>\n                    Inflammasome"],"prefix":"10.1002","volume":"14","author":[{"given":"Chunting","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Nutrition, School of Public Health Dali University  Dali 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