{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T08:33:04Z","timestamp":1793003584339,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science &amp; Nutrition"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This research aimed to investigate the hepatoprotective and anti\u2010senescence effects of Nongxiang\u2010type Tieguanyin (Tgy\u2010N) on D\u2010galactose (D\u2010gal)\u2010induced aging C57BL\/6 mice and to elucidate the underlying mechanisms. The chemical composition of Tgy\u2010N was characterized by colorimetric assays and HPLC. An aging mouse model was established by daily intraperitoneal injection of D\u2010gal (200\u2009mg\/kg) for 8\u2009weeks, with simultaneous intragastric administration of Tgy\u2010N at 110 or 550\u2009mg\/kg\/day. Behavioral tests, serum biochemical analyses, hepatic oxidative stress markers, histopathological examination, transcriptomic profiling, and western blotting were performed. The anti\u2010senescence effect was further validated in Nutlin\u20103a\u2010induced senescent AML12 and HepG2 cells. Tgy\u2010N contained abundant tea polyphenols (286.91\u2009mg\/g) and catechins (137.89\u2009mg\/g). HPLC analysis with authentic external standards revealed that the absolute contents of EGCG, catechin, catechin gallate, gallic acid, and epigallocatechin in Tgy\u2010N extract were 7.96, 5.79, 4.06, 1.67, and 4.91\u2009mg\/g, respectively. In\u00a0vivo, Tgy\u2010N significantly improved exercise endurance and energy metabolism, reduced the hepatosomatic index, attenuated hepatic oxidative stress (elevated SOD and GSH, reduced MDA), and decreased serum ALT and AST levels. Histologically, Tgy\u2010N ameliorated hepatic lobule disorganization, reduced fibrosis, decreased SA\u2010\u03b2\u2010gal\u2010positive areas, downregulated P16\/P21\/P53, and upregulated Sirt1. Transcriptomic and western blot analyses revealed that Tgy\u2010N suppressed the NF\u2010\u03baB signaling pathway by reducing phosphorylation of IKK\u03b1\/\u03b2 and p65. Cellular experiments confirmed that Tgy\u2010N reduced p16\/p21 expression and SA\u2010\u03b2\u2010gal\u2010positive cell numbers in Nutlin\u20103a\u2010induced senescent hepatocytes. Tgy\u2010N protects against D\u2010gal\u2010induced hepatic senescence through oxidative stress mitigation and NF\u2010\u03baB pathway inhibition, supporting its potential as a natural functional food for liver health.<\/jats:p>","DOI":"10.1002\/fsn3.72396","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:10:52Z","timestamp":1790683852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective Effects of Nongxiang\u2010Type\n                    <i>Tieguanyin<\/i>\n                    on the Liver of D\u2010Galactose\u2010Induced Aging\n                    <scp>C57BL<\/scp>\n                    \/6 Mice via the\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    Signaling Pathway"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6150-5112","authenticated-orcid":false,"given":"Weihong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Central Laboratory Anxi County Hospital  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingmei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Anxi County Hospital  Quanzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxiao","family":"Ye","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences Xiamen University  Xiamen Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Central Laboratory Anxi County Hospital  Quanzhou Fujian 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