{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:04:56Z","timestamp":1791561896401,"version":"4.3.4"},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:00:00Z","timestamp":1542585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Food Science & Nutrition"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Increasing use of medicinal herbs as nutritional supplements and traditional medicines for the treatment of diabetes, hypertension, hyperlipidemia, and malaria fever with conventional drugs poses possibilities of herb\u2013drug interactions (\n HDI<\/jats:styled-content>\n s). The potential of nine selected widely used tropical medicinal herbs in inhibiting human cytochrome P450 (\n CYP<\/jats:styled-content>\n ) isoenzymes was investigated.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and methods<\/jats:title>\n \n In vitro inhibition of eight major\n CYP<\/jats:styled-content>\n isoenzymes by aqueous extracts of\n Allium sativum, Gongronema latifolium, Moringa oleifera<\/jats:italic>\n ,\n Musa sapientum, Mangifera indica, Tetracarpidium conophorum<\/jats:italic>\n ,\n Alstonia boonei, Bauhinia monandra,<\/jats:italic>\n and\n Picralima nitida<\/jats:italic>\n was estimated in human liver microsomes by monitoring twelve probe metabolites of nine probe substrates with\n UPLC<\/jats:styled-content>\n \/\n MS<\/jats:styled-content>\n \u2010\n MS<\/jats:styled-content>\n using validated N\u2010in\u2010one assay method.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Mangifera indica<\/jats:italic>\n moderately inhibited\n CYP<\/jats:styled-content>\n 2C8,\n CYP<\/jats:styled-content>\n 2B6,\n CYP<\/jats:styled-content>\n 2D6,\n CYP<\/jats:styled-content>\n 1A2, and\n CYP<\/jats:styled-content>\n 2C9 with\n IC<\/jats:styled-content>\n 50<\/jats:sub>\n values of 37.93, 57.83, 67.39, 54.83, and 107.48\u00a0\u03bcg\/\n ml<\/jats:styled-content>\n , respectively, and\n Alstonia boonei<\/jats:italic>\n inhibited\n CYP<\/jats:styled-content>\n 2D6 (\n IC<\/jats:styled-content>\n 50<\/jats:sub>\n \u00a0=\u00a077.19\u00a0\u03bcg\/\n ml<\/jats:styled-content>\n ).\n Picralima nitida<\/jats:italic>\n inhibited\n CYP<\/jats:styled-content>\n 3A4 (\n IC<\/jats:styled-content>\n 50<\/jats:sub>\n \u00a0=\u00a045.58\u00a0\u03bcg\/\n ml<\/jats:styled-content>\n ) and\n CYP<\/jats:styled-content>\n 2C19 (\n IC<\/jats:styled-content>\n 50<\/jats:sub>\n \u00a0=\u00a073.06\u00a0\u03bcg\/\n ml<\/jats:styled-content>\n ) moderately but strongly inhibited\n CYP<\/jats:styled-content>\n 2D6 (\n IC<\/jats:styled-content>\n 50<\/jats:sub>\n \u00a0=\u00a01.19\u00a0\u03bcg\/\n ml<\/jats:styled-content>\n ). Other aqueous extracts of\n Gongronema latifolium, Bauhinia monandra,<\/jats:italic>\n and\n Moringa oleifera<\/jats:italic>\n showed weak inhibitory activities against\n CYP<\/jats:styled-content>\n 1A2.\n Musa sapientum, Allium sativum,<\/jats:italic>\n and\n Tetracarpidium conophorum<\/jats:italic>\n did not inhibit the\n CYP<\/jats:styled-content>\n isoenzymes investigated.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Potential for clinically important\n CYP<\/jats:styled-content>\n \u2010metabolism\u2010mediated\n HDI<\/jats:styled-content>\n s is possible for\n Alstonia boonei, Mangifera indica,<\/jats:italic>\n and\n Picralima nitida<\/jats:italic>\n with drugs metabolized by\n CYP<\/jats:styled-content>\n 2C8, 2B6, 2D6, 1A2, 2C9, 2C19, and 3A4. Inhibition of\n CYP<\/jats:styled-content>\n 2D6 by\n Picralima nitida<\/jats:italic>\n is of particular concern and needs immediate in vivo investigations.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/fsn3.789","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T21:39:47Z","timestamp":1542663587000},"page":"44-55","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Potential inhibition of major human cytochrome P450 isoenzymes by selected tropical medicinal herbs\u2014Implication for herb\u2013drug interactions"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3748-091X","authenticated-orcid":false,"given":"Segun Johnson","family":"Showande","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy Department of Clinical Pharmacy and Pharmacy Administration University of Ibadan Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Titilayo Oyelola","family":"Fakeye","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Department of Clinical Pharmacy and Pharmacy Administration University of Ibadan Ibadan Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marena","family":"Kajula","sequence":"additional","affiliation":[{"name":"Admescope Ltd Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juho","family":"Hokkanen","sequence":"additional","affiliation":[{"name":"Admescope Ltd Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ari","family":"Tolonen","sequence":"additional","affiliation":[{"name":"Admescope Ltd Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/587160"},{"key":"e_1_2_9_3_1","first-page":"614","article-title":"Inhibition potential of Moringa oleifera Lam. on drug metabolizing enzymes","volume":"14","author":"Ahmmed S. 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