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Therefore, this study used the spontaneously hypertensive rats (SHR) hypertension model and the isolated rabbit thoracic aorta rings model to explore the mechanisms underlying the hypotension and vasorelaxation effects of RJ. Rats were divided into the following groups (<jats:italic>n<\/jats:italic>\u00a0=\u00a06): WKY\u2010control group, SHR\u2010control group, and SHR\u2010RJ group. SHR\u2010RJ group was received 1\u00a0g\/kg of RJ via oral administration daily for 4\u00a0weeks. Systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), and nitric oxide (NO) level were detected. In addition, the mechanism of vasodilation of RJ was investigated using an isolated rabbit aortic ring technique. RJ significantly reduced SBP and DBP as well as increased NO levels of SHR in vivo. RJ caused vasorelaxation of the isolated aorta rings, and this effect was inhibited by atropine (M<jats:sub>3<\/jats:sub> receptor blocker), L\u2010NAME (nitric oxide synthase inhibitor), methylene blue (guanylate cyclase inhibitor), and indomethacin (cyclooxygenase inhibitor). Moreover, RJ could markedly suppress the NE\u2010induced intracellular Ca<jats:sup>2+<\/jats:sup> releases and high K<jats:sup>+<\/jats:sup>\u2010induced extracellular Ca<jats:sup>2+<\/jats:sup> influx in denuded aortic rings. In addition, RJ can also increase cGMP levels and the production of NO in isolated aortic rings. The present study showed that RJ has antihypertensive effects and was associated with increased NO production. In addition, RJ contains muscarinic receptor agonist, possibly an acetylcholine\u2010like substance, and induces vasodilation through NO\/cGMP pathway and calcium channels.<\/jats:p>","DOI":"10.1002\/fsn3.970","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T02:30:14Z","timestamp":1550457014000},"page":"1361-1370","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Royal jelly causes hypotension and vasodilation induced by increasing nitric oxide production"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8085-9760","authenticated-orcid":false,"given":"Yongming","family":"Pan","sequence":"first","affiliation":[{"name":"College of Animal Sciences Zhejiang University Hangzhou China"},{"name":"Comparative Medical Research Institute, Experimental Animal Research Center Zhejiang Chinese Medical University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yili","family":"Rong","sequence":"additional","affiliation":[{"name":"Comparative Medical Research Institute, Experimental Animal Research Center Zhejiang Chinese Medical University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengmeng","family":"You","sequence":"additional","affiliation":[{"name":"College of Animal Sciences Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quanxin","family":"Ma","sequence":"additional","affiliation":[{"name":"Comparative Medical Research Institute, Experimental Animal Research Center Zhejiang Chinese Medical University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minli","family":"Chen","sequence":"additional","affiliation":[{"name":"Comparative Medical Research Institute, Experimental Animal Research Center Zhejiang Chinese Medical University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuliang","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Animal Sciences Zhejiang University Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.09.057"},{"issue":"2","key":"e_1_2_9_3_1","first-page":"520","article-title":"Norepinephrine\u2010induced relaxations in rat aorta mediated by endothelial beta adrenoceptors. 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