{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T19:19:36Z","timestamp":1788376776088,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,8,30]],"date-time":"2002-08-30T00:00:00Z","timestamp":1030665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Research Fund of Tampere University Hospital"},{"name":"Finnish Medical Society Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In breast cancer, several chromosomal sites frequently undergo amplification, implicating the location of genes important for tumor development and progression. Here we cloned two novel genes, breast carcinoma amplified sequence 3 (\n                    <jats:italic>BCAS3<\/jats:italic>\n                    ) and 4 (\n                    <jats:italic>BCAS4<\/jats:italic>\n                    ), from the two most common amplification sites in breast cancer, 17q23 and 20q13. The\n                    <jats:italic>BCAS3<\/jats:italic>\n                    gene at 17q23 spans more than 600 kb at the genomic level and was predicted to encode a 913 amino acid nuclear protein. The\n                    <jats:italic>BCAS4<\/jats:italic>\n                    gene at 20q13.2 encodes a 211 amino acid cytoplasmic protein. Both\n                    <jats:italic>BCAS3<\/jats:italic>\n                    and\n                    <jats:italic>BCAS4<\/jats:italic>\n                    represent novel genes with no homologies to any other known gene or protein. In the MCF7 breast cancer cell line, the\n                    <jats:italic>BCAS3<\/jats:italic>\n                    and\n                    <jats:italic>BCAS4<\/jats:italic>\n                    genes were co\u2010amplified, and cloning of a highly overexpressed 1.3\u2010kb transcript revealed a rearrangement fusing the last two exons of\n                    <jats:italic>BCAS3<\/jats:italic>\n                    with\n                    <jats:italic>BCAS4.<\/jats:italic>\n                    The fusion led to a novel message in which only the first exon of\n                    <jats:italic>BCAS4<\/jats:italic>\n                    and part of exon 23 of\n                    <jats:italic>BCAS3<\/jats:italic>\n                    were transcribed. The\n                    <jats:italic>BCAS4\u2013BCAS3<\/jats:italic>\n                    fusion transcript was detected only in MCF7 cells, but the\n                    <jats:italic>BCAS4<\/jats:italic>\n                    gene was also overexpressed in nine of 13 breast cancer cell lines. In conclusion, our results indicate that these novel genes,\n                    <jats:italic>BCAS3<\/jats:italic>\n                    at 17q23 and\n                    <jats:italic>BCAS4<\/jats:italic>\n                    at 20q13.2, undergo amplification, overexpression, and fusion in breast cancer and therefore may have a role in the frequent chromosomal alterations affecting these two loci. Published 2002 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/gcc.10121","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T11:27:43Z","timestamp":1034767663000},"page":"311-317","source":"Crossref","is-referenced-by-count":99,"title":["Cloning of\n                    <i>BCAS3<\/i>\n                    (17q23) and\n                    <i>BCAS4<\/i>\n                    (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer\u2020"],"prefix":"10.1002","volume":"35","author":[{"given":"Maarit","family":"B\u00e4rlund","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Outi","family":"Monni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Donald","family":"Weaver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e4ivikki","family":"Kauraniemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Sauter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mervi","family":"Heiskanen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olli\u2010P.","family":"Kallioniemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Kallioniemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,8,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20011001)45:2<83::AID-CYTO1149>3.0.CO;2-P"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64158-2"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5328.965"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.15.1252"},{"key":"e_1_2_5_6_1","first-page":"5340","article-title":"Multiple 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