{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T08:36:05Z","timestamp":1790152565710,"version":"4.0.1"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,9,15]],"date-time":"2002-09-15T00:00:00Z","timestamp":1032048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The large zinc finger protein KRC binds to the signal sequences of V(D)J recombination and the \u03baB motif. Disruption of KRC expression in cell lines resulted in increased cell proliferation, anchorage independence of growth, and uncoupling of nuclear division and cell division. In this report, the function of KRC was studied in a RAG2\u2010deficient blastocyst complementation animal model. KRC\u2010deficient embryonic stem cells were generated by homologous recombination and were introduced into <jats:italic>RAG2<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> blastocysts to generate <jats:italic>KRC<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup><jats:italic>;RAG2<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> chimeric mice. The lymphoid compartments of chimeras examined at 5 weeks of age were developed, suggesting that KRC is not essential for V(D)J recombination development. However, by 6 months of age, there was a marked deficit in CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> thymocytes in the chimeras, suggesting that KRC may be involved in T\u2010lymphocyte survival. Additionally, one chimera developed anomalies, including postaxial polydactyly, hydronephrosis, and an extragonadal malignant teratoma. DNA analysis showed that the teratoma was derived from <jats:italic>KRC<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> embryonic stem cells. The teratoma had compound tissue organization and was infiltrated with B lymphocytes. Subsequently, several immortalized KRC\u2010deficient cell lines were established from the teratoma. In this study, growth anomalies and neoplasia were observed in animals and cells deficient in KRC, and other studies have shown allelic loss occurring at the chromosomal region of the human KRC counterpart in various tumors. We propose that <jats:italic>KRC<\/jats:italic> may be a previously unidentified tumor\u2010suppresser gene. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.10128","type":"journal-article","created":{"date-parts":[[2002,10,16]],"date-time":"2002-10-16T15:27:43Z","timestamp":1034782063000},"page":"287-298","source":"Crossref","is-referenced-by-count":14,"title":["Developmental anomalies and neoplasia in animals and cells deficient in the large zinc finger protein KRC"],"prefix":"10.1002","volume":"35","author":[{"given":"Carl E.","family":"Allen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natarajan","family":"Muthusamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven E.","family":"Weisbrode","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joung Woo","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lai\u2010Chu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,9,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2000.5029"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90539-1"},{"key":"e_1_2_5_4_1","first-page":"584","article-title":"Spontaneous extragonadal teratocarcinoma in a mouse","volume":"28","author":"Artzt K","year":"1978","journal-title":"Lab Anim Sci"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11991"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.4.1406"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(98)00143-8"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(99)00249-6"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1074\/jbc.270.3.1221","article-title":"Murine transcription factor \u03b1\u2010A crystallin binding protein I","volume":"270","author":"Brady JP","year":"1995","journal-title":"J Biol Chem"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.10.4528"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.16.3335"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90362-4"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9901"},{"key":"e_1_2_5_14_1","first-page":"644","article-title":"Genetics and biology of human ovarian teratomas. 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