{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:14:44Z","timestamp":1790396084663,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,9,15]],"date-time":"2002-09-15T00:00:00Z","timestamp":1032048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Telomerase, the ribonucleoprotein enzyme that maintains the telomere, is active in human germ and stem cells and in a majority of tumor tissues and immortalized cell lines. In contrast, telomerase activity is not detected in most somatic cells, suggesting that normal human cells contain a regulatory factor(s) to repress this activity. To identify which human chromosomes carry a gene or genes that function as telomerase repressors, we investigated telomerase activity in hybrids of the B16\u2010F10 cell line, which contain individual human chromosomes transferred previously by microcell fusion and therefore represent a hybrid panel for the entire genome except for the Y chromosome. Microcell hybrids with an introduced normal human chromosome 5 showed inhibition of telomerase activity, but clones at a late passage exhibited reactivation of telomerase activity. Reactivation of telomerase activity was accompanied by deletion and\/or rearrangement of the transferred human chromosome 5. The introduction of other human chromosomes did not significantly affect the telomerase activity of B16\u2010F10 cells. The effect of suppression of telomerase activity in microcell hybrids containing chromosome 5 was accompanied by a reduction in the level of <jats:italic>mTERT<\/jats:italic> mRNA, which encodes a component of the telomerase complex. The putative telomerase repressor gene was mapped to human chromosome bands 5p11\u2013p13 by a combination of functional analysis using transfer of subchromosomal transferable fragments of chromosome 5 into B16\u2010F10 cells and deletion mapping of revertant clones with reactivated telomerase activity. Thus, these results suggest that loss of a gene(s) on this chromosome was responsible for telomerase reactivation, indicating that human chromosome 5 contains a gene or genes that can regulate the expression of <jats:italic>mTERT<\/jats:italic> in B16\u2010F10 cells. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gcc.10135","type":"journal-article","created":{"date-parts":[[2002,12,3]],"date-time":"2002-12-03T13:50:22Z","timestamp":1038923422000},"page":"37-47","source":"Crossref","is-referenced-by-count":23,"title":["Human chromosome 5 carries a putative telomerase repressor gene"],"prefix":"10.1002","volume":"36","author":[{"given":"Hiroyuki","family":"Kugoh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyo","family":"Shigenami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Funaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Carl","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuo","family":"Oshimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11,27]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"91","article-title":"A preneoplastic stage in the spontaneous neoplastic transformation of Syrian hamster embryo cells in culture","volume":"40","author":"Barrett JC","year":"1980","journal-title":"Cancer Res"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.8.3761"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)80006-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.349"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90443-7"},{"key":"e_1_2_6_7_1","first-page":"893","article-title":"Telomerase activity in normal and malignant murine tissues","volume":"11","author":"Chadeneau C","year":"1995","journal-title":"Oncogene"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05245.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.8.2900"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio242148a0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.13.2557"},{"key":"e_1_2_6_12_1","first-page":"2116","article-title":"Expression of the hTERT gene is regulated at the level of transcriptional initiation repressed by Mad1","volume":"60","author":"Gunes C","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/345458a0"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0395-249"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199806)22:2<65::AID-MC1>3.0.CO;2-J"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s100380170101"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7605428"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/347400a0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940020103"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8469989"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/6.3.165"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(90)90477-C"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(92)90160-T"},{"key":"e_1_2_6_24_1","first-page":"669","article-title":"The transition from primary culture to spontaneous immortalization in mouse fibroblast populations","volume":"8","author":"Macieira\u2010Coelho A","year":"1988","journal-title":"Anticancer Res"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80538-3"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4619"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5328.955"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/304648a0"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.13.5635"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204165"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.10.6036"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.52.37473"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1995.tb02998.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6879195"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.11.4818"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.4.1493"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202261"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.12.5498"},{"key":"e_1_2_6_39_1","first-page":"6090","article-title":"Evidence for multiple pathways to cellular senescence","volume":"54","author":"Sasaki M","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.11.865"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2300822"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199810)23:2<123::AID-GCC5>3.0.CO;2-4"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870140206"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199805)22:1<34::AID-MC5>3.0.CO;2-L"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70109-5"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.18.3794"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940090303"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.10135","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.10135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T14:12:13Z","timestamp":1697465533000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.10135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11,27]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,1]]}},"alternative-id":["10.1002\/gcc.10135"],"URL":"https:\/\/doi.org\/10.1002\/gcc.10135","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11,27]]}}}